Stress proteins accumulate in cultured retinal glial cells during herpes simplex viral infection

The production of stress- or heat-shock proteins (SP) which are defined by three monoclonal antibodies (TI56, TG5E and TG7A) were examined in cultured retinal glial cells with and without herpes simplex virus (HSV) infection. Indirect immunofluorescence showed that 80-90% of uninfected cells reacted with anti-glial fibrillary acidic protein (GFAP) and that 10-20% of uninfected cells were weakly labelled with anti-SP antibodies. By 6 hr after HSV infection, the proportion of GFAP labelled cells decreased to 60-70% whereas cells strongly expressing SP antigens were demonstrated. At 24 hr, GFAP+ cells were markedly reduced in number and immunolabelling with anti-SP antibodies was evident in approximately 50% of cells, directly demonstrating the accumulation of SP in cultured retinal cells after HSV infection. Double labelling with GFAP/TI56 indicated that 30% of GFAP+ cells were labelled with TI56 and 30-50% of TI56+ cells were also GFAP+, despite the abrupt loss of GFAP+ cells during HSV infection. These results indicate that SP normally expressed at low level are significantly upregulated in retinal glial cells following HSV infection.