Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Two-Dimensional Microscopy in Microbiology

Two-Dimensional Microscopy in Microbiology

Two-dimensional (2D) microscopy encompasses a range of optical techniques that capture images within a single focal plane, offering detailed representations of microscopic structures. These techniques are essential in biological and medical research, enabling the visualization of cellular and subcellular structures with different levels of contrast and specificity.There are several major types of 2D microscopy, each with strengths and applications.Bright-Field MicroscopyBright-field microscopy...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Exploring the intricacies of antigen-antibody interfaces: Effects of binding-induced conformational changes and distinct interaction patterns.

International journal of biological macromolecules·2026

Same author

Detection of ongoing asymptomatic <i>Porcine deltacoronavirus</i> infections via transiently produced IgGs using protein-peptide hybrid microarray.

Frontiers in microbiology·2026

Same author

Plasma miRNA signature for the diagnosis of pulmonary tuberculosis in symptomatic patients.

Thorax·2026

Same author

Remote medical system driven by medical big models: Dynamic defense model for network security threats.

PloS one·2026

Same author

Combining receptor engineering and intracellular gelation for cell-based antiviral therapy against coxsackievirus B3.

Journal of nanobiotechnology·2026

Same author

Comprehensive evaluation of the triglyceride glucose index (TyG) and body roundness index (BRI) on cardiovascular disease risk prediction: a 9-year prospective cohort study in Chinese middle-aged and older adults.

Cardiovascular diabetology·2026

Search research articles

Related Experiment Video

Updated: Mar 6, 2026

Author Spotlight: Single-Molecule Surface-Enhanced Raman Scattering Measurements Enabled by Plasmonic DNA Origami Nanoantennas

Author Spotlight: Single-Molecule Surface-Enhanced Raman Scattering Measurements Enabled by Plasmonic DNA Origami Nanoantennas

Published on: July 21, 2023

MicroRNA Biosensing with Two-Dimensional Surface Plasmon Resonance Imaging.

Ho Pui Ho¹, Fong Chuen Loo², Shu Yuen Wu²

¹Department of Electronic Engineering, Center for Advanced Research in Photonics, The Chinese University of Hong Kong, Room 404, Ho Sin Hang Engineering Building, N.T. Hong Kong SAR, China. hpho@ee.cuhk.edu.hk.

Methods in Molecular Biology (Clifton, N.J.)

|March 11, 2017

Summary

A novel MicroRNA-RNase-SPR (MARS) assay enhances biosensing for microRNA detection. This method amplifies signals for rapid influenza A screening without PCR pre-amplification.

Keywords:

Biosensing Disease screening Influenza MicoRNA-RNase-SPR MicroRNA Signal amplification Two-dimensional surface plasmon resonance

More Related Videos

Multimodal Analytical Platform on a Multiplexed Surface Plasmon Resonance Imaging Chip for the Analysis of Extracellular Vesicle Subsets

Multimodal Analytical Platform on a Multiplexed Surface Plasmon Resonance Imaging Chip for the Analysis of Extracellular Vesicle Subsets

Published on: March 17, 2023

A Label-free Technique for the Spatio-temporal Imaging of Single Cell Secretions

A Label-free Technique for the Spatio-temporal Imaging of Single Cell Secretions

Published on: November 23, 2015

Related Experiment Videos

Last Updated: Mar 6, 2026

Author Spotlight: Single-Molecule Surface-Enhanced Raman Scattering Measurements Enabled by Plasmonic DNA Origami Nanoantennas

Author Spotlight: Single-Molecule Surface-Enhanced Raman Scattering Measurements Enabled by Plasmonic DNA Origami Nanoantennas

Published on: July 21, 2023

Multimodal Analytical Platform on a Multiplexed Surface Plasmon Resonance Imaging Chip for the Analysis of Extracellular Vesicle Subsets

Multimodal Analytical Platform on a Multiplexed Surface Plasmon Resonance Imaging Chip for the Analysis of Extracellular Vesicle Subsets

Published on: March 17, 2023

A Label-free Technique for the Spatio-temporal Imaging of Single Cell Secretions

A Label-free Technique for the Spatio-temporal Imaging of Single Cell Secretions

Published on: November 23, 2015

Area of Science:

Biomedical Engineering
Molecular Diagnostics
Surface Plasmon Resonance Imaging

Background:

Two-dimensional surface plasmon resonance (2D-SPR) imaging offers sensitive, label-free, real-time analysis of biomolecular interactions.
Direct detection of low-abundance microRNAs in clinical samples using 2D-SPR is challenging due to weak signals.

Purpose of the Study:

To develop an efficient biosensing technique for amplifying microRNA signals.
To enable rapid screening of influenza A (H1N1) using microRNA detection.

Main Methods:

The MicroRNA-RNase-SPR (MARS) assay was developed, utilizing RNase H for enzymatic digestion of microRNA probes.
This enzymatic digestion amplifies the 2D-SPR signal, increasing readout speed.
The assay was applied to detect microRNA hsa-miR-29a-3p, a biomarker for influenza infection.

Main Results:

The MARS assay demonstrated significant signal amplification for microRNA detection.
The technique achieved faster readout speeds compared to conventional methods.
Successful application in targeting microRNA associated with influenza A (H1N1) infection.

Conclusions:

The MARS assay provides a robust and rapid method for microRNA detection and signal amplification.
This technique overcomes the limitations of low SPR signals for direct microRNA analysis.
The MARS assay shows potential for rapid clinical screening of infectious diseases like influenza.