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Related Concept Videos

DNA Microarrays02:34

DNA Microarrays

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Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...
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Labeling DNA Probes03:31

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DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
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Related Experiment Video

Updated: Mar 6, 2026

Multimodal Analytical Platform on a Multiplexed Surface Plasmon Resonance Imaging Chip for the Analysis of Extracellular Vesicle Subsets
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Gold Nanorod Array Biochip for Label-Free, Multiplexed Biological Detection.

Zhong Mei1, Yanyan Wang1, Liang Tang2

  • 1Department of Biomedical Engineering, University of Texas at San Antonio, One UTSA Circle, San Antonio, TX, 78249, USA.

Methods in Molecular Biology (Clifton, N.J.)
|March 11, 2017
PubMed
Summary

This study presents a stable, chip-based gold nanorod (GNR) biosensor for sensitive, label-free detection of biomarkers. The GNR array enables high-throughput, multiplexed analysis of proteins and DNA, overcoming aggregation issues of solution-based sensors.

Keywords:
BiochipGold nanorodsMultiplexSurface plasmon resonance

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Area of Science:

  • Nanotechnology
  • Biomedical Engineering
  • Optical Sensing

Background:

  • Gold nanorods (GNRs) offer sensitive label-free sensing via localized surface plasmon resonance (LSPR).
  • Solution-based GNR sensors face challenges like aggregation, leading to unstable readings and short shelf life.
  • A robust, chip-based platform is needed for reliable GNR biosensing.

Purpose of the Study:

  • To develop a stable, chip-based GNR biosensor for sensitive and multiplexed label-free detection.
  • To immobilize functionalized GNRs on a glass substrate for enhanced reliability.
  • To leverage GNRs' aspect-ratio-dependent LSPR for simultaneous analyte detection.

Main Methods:

  • Functionalized GNRs were immobilized on a (3-mercaptopropyl)trimethoxysilane modified glass substrate via covalent Au-S bonds.
  • GNRs with varying aspect ratios were patterned onto designated spots for distinct LSPR peaks.
  • Spectral shifts of LSPR peaks upon biological binding were monitored using a microplate reader.

Main Results:

  • The developed biochip demonstrated high sensitivity and stability in physiological buffer.
  • The chip-based format prevented nanoparticle aggregation, ensuring reliable measurements.
  • Simultaneous detection of specific analytes was achieved through spatially resolved GNR arrays.

Conclusions:

  • The chip-based GNR biosensor offers a robust and reliable platform for label-free detection.
  • This technology enables high-throughput and multiplexed analysis of biological samples.
  • The GNR array biosensor is effective for protein/DNA array analysis and disease biomarker detection.