Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

The SV40 enhancer can be dissected into multiple segments, each with a different cell type specificity.

S Schirm1, J Jiricny, W Schaffner

  • 1Institute für Molekularbiologie II der Universität Zürich, Switzerland.

Genes & Development
|March 1, 1987
PubMed
Summary

Individual segments of the Simian virus 40 (SV40) enhancer show distinct cell-type specific activities. Combining these motifs allows for the assembly of enhancers with varied specificities, revealing key sequence elements.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Prime editing efficiency and fidelity are enhanced in the absence of mismatch repair.

Nature communications·2022
Same author

Comparison and modelling of pegylated or unpegylated G-CSF schedules in CHOP-14 regimen of elderly patients with aggressive B-cell lymphoma.

Annals of hematology·2013
Same author

Epigenetic silencing of monoallelically methylated miRNA loci in precancerous colorectal lesions.

Oncogenesis·2013
Same author

Unique mutational profile associated with a loss of TDG expression in the rectal cancer of a patient with a constitutional PMS2 deficiency.

DNA repair·2012
Same author

Estimating the undetected burden of influenza hospitalizations in children.

Epidemiology and infection·2006
Same author

Novel and recurrent germline alterations in the MLH1 and MSH2 genes identified in hereditary nonpolyposis colorectal cancer patients in Slovakia.

Neoplasma·2006

Area of Science:

  • Molecular Biology
  • Genetics
  • Cell Biology

Background:

  • The Simian virus 40 (SV40) enhancer is a regulatory DNA sequence crucial for gene expression, known for its broad activity across various tissues and species.
  • It comprises multiple protein-binding sequence motifs essential for its function.
  • Understanding the contribution of individual motifs to enhancer activity and specificity is key to deciphering gene regulation.

Purpose of the Study:

  • To investigate the enhancer activity and cell-type specificity of individual sequence motifs within the SV40 enhancer.
  • To determine if short DNA segments can function as enhancers independently.
  • To identify critical sequence elements responsible for the SV40 enhancer's activity and specificity.

Main Methods:

  • Synthesis and multimerization of three distinct SV40 enhancer-derived oligonucleotides: corePVUII, coreC, and SPHI.

Related Experiment Videos

  • Linking these multimerized oligonucleotides to a beta-globin reporter gene.
  • Transient expression assays in 10 diverse cell lines to analyze reporter gene transcription.

    • Main Results:

    • Each of the three synthetic oligonucleotide segments demonstrated enhancer activity when multimerized.
    • None of the tested enhancer segments exhibited ubiquitous activity; each was active in specific cell subpopulations.
    • The core sequence variants displayed the most pronounced cell-type specificity, with one segment mimicking the activity profile of the entire SV40 enhancer.

    Conclusions:

    • Individual sequence motifs within the SV40 enhancer possess inherent cell-type specific regulatory potential.
    • The cell-type specificity of the SV40 enhancer arises from the combinatorial activity of its constituent sequence elements.
    • These findings highlight the importance of specific sequence motifs and their arrangement in determining enhancer function and tissue-specific gene expression.