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Convenient vectors for cloning and sequencing EcoRI and HindIII fragments
H J Edenberg1, L G Moss, W J Rutter
1Hormone Research Institute, University of California, San Francisco 94143-0534.
Gene
|January 1, 1987
Abstract:
The polylinker regions of plasmid pUC and bacteriophage M13mp vectors have been specifically modified to provide alternative positions for cloning and reexcising EcoRI and HindIII fragments; the EcoRI and HindIII sites have been moved internal to BamHI and Bg/II sites. The location of EcoRI and HindIII sites in these HinEco vectors allows either selective linearization or excision of the cloned fragments at unique flanking sites.