Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

ATP Synthase: Mechanism01:48

ATP Synthase: Mechanism

18.1K
In animals, the mitochondrial F1F0 ATP synthase is the key protein that synthesizes ATP molecules through a complex catalytic mechanism. While the nuclear genome encodes the majority of ATP synthase subunits, the mitochondrial genome encodes some of the enzyme's most critical components. The formation of this multi-subunit enzyme is a complex multi-step process regulated at the level of transcription, translation, and assembly. Defects in one or more of these steps can result in decreased...
18.1K
Allosteric Proteins-ATCase01:19

Allosteric Proteins-ATCase

6.8K
Binding sites linkages can regulate a protein's function.  For example, enzyme activity is often regulated through a feedback mechanism where the end product of the biochemical process serves as an inhibitor.
Aspartate transcarbamoylase (ATCase) is a cytosolic enzyme that catalyzes the condensation of L-aspartate and carbamoyl phosphate to  N-carbamoyl-L-aspartate. This reaction is the first step in pyrimidine biosynthesis. UTP and CTP, the end products of the pyrimidine synthesis...
6.8K
ATP Synthase: Structure01:18

ATP Synthase: Structure

16.6K
ATP synthase or ATPase is among the most conserved proteins found in bacteria, mammals, and plants. This enzyme can catalyze a forward reaction in response to the electrochemical gradient, producing ATP from ADP and inorganic phosphate. ATP synthase can also work in a reverse direction by hydrolyzing ATP and generating an electrochemical gradient. Different forms of ATP synthases have evolved special features to meet the specific demands of the cell. Based on their specific feature, ATP...
16.6K
Gene Regulation During Sporulation01:17

Gene Regulation During Sporulation

585
Sporulation is a complex developmental process that allows certain Gram-positive bacteria, such as Bacillus subtilis and Clostridium species, to survive extreme environmental conditions. This process is tightly regulated by a series of signaling cascades and transcriptional controls, ensuring the formation of a highly resistant endospore.Sporulation is triggered by unfavorable conditions, such as nutrient depletion, and is governed by a phosphorelay system. One of the sensor kinases, such as...
585
Spindle Assembly02:50

Spindle Assembly

4.4K
Spindle assembly occurs through three, often coexisting, pathways – the centrosome-mediated pathway, the chromatin-mediated pathway, and the microtubule-mediated pathway – collectively contributing to form a robust spindle apparatus.
In most cells, centrosomes are the primary microtubule nucleation centers. In the centrosome-mediated pathway, the G2-prophase transition triggers centrosome maturation and increased microtubule nucleation. Progressive nucleation results in a...
4.4K
Membrane Asymmetry Regulating Transporters01:19

Membrane Asymmetry Regulating Transporters

7.7K
Enzymes like flippase, floppase, and scramblase transfer phospholipids from one layer to another in the membrane, thereby affecting membrane asymmetry.
Flippase
Eukaryotic flippases are type-IV P-type ATPases or P4-ATPases belonging to P-type ATPase family proteins that are membrane-bound pumps involved in the ATP-mediated transport of ions and molecules across the membrane. Flippases flip specific phospholipids from the outer to the inner leaflet of a membrane. All P4-ATPases have one...
7.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Reduced GABA Levels in the ACC of Actively Drinking High Risk Individuals Compared to Recently Detoxified Alcohol-Dependent Patients.

Journal of integrative neuroscience·2024
Same author

Correlation of striatal dopamine D2/3 receptor availability with GABA level in the anterior cingulate cortex in healthy controls but not in alcohol-dependent subjects and individuals at high risk: A multimodal magnetic resonance spectroscopy and positron emission tomography study.

Addiction biology·2024
Same author

Small molecule inhibitors for cancer immunotherapy and associated biomarkers - the current status.

Frontiers in immunology·2023
Same author

Liquid scintillation counting at the limit of detection in biogeosciences.

Frontiers in microbiology·2023
Same author

Efficacy of Adjuvants in Ophthalmic Regional Anesthesia: A Systematic Review and Network Meta-analysis.

American journal of ophthalmology·2023
Same author

SPATA2 restricts OTULIN-dependent LUBAC activity independently of CYLD.

Cell reports·2023
Same journal

Multi-omic single nuclei profiling of murine pancreas shows dynamic epigenetic heterogeneity of acinar cells.

Cell death and differentiation·2026
Same journal

A planar dimer of bovine ATP synthase.

Cell death and differentiation·2026
Same journal

GCN5 and TADA2B constitutively regulate XRCC1 function during DNA repair to maintain cell survival.

Cell death and differentiation·2026
Same journal

MEGF8 controls osteogenic differentiation through post-transcriptional regulation of BMP-SMAD signaling in craniosynostosis.

Cell death and differentiation·2026
Same journal

Macrophage-secreted brain-derived neurotrophic factor promotes tumor growth in triple-negative breast cancer by inducing axonogenesis.

Cell death and differentiation·2026
Same journal

Species-specific regulation of necroptosis by STK38-dependent RIPK1 phosphorylation.

Cell death and differentiation·2026
See all related articles

Related Experiment Video

Updated: Mar 6, 2026

Reconstitution of Msp1 Extraction Activity with Fully Purified Components
05:52

Reconstitution of Msp1 Extraction Activity with Fully Purified Components

Published on: August 10, 2021

3.0K

SPATA2: more than a missing link.

Lisa Schlicher1,2,3, Prisca Brauns-Schubert1,2,3, Florian Schubert1,2,3

  • 1Institute of Molecular Medicine and Cell Research, Albert-Ludwigs-University Freiburg, Stefan Meier Strasse 17, Freiburg 79104, Germany.

Cell Death and Differentiation
|March 11, 2017
PubMed
Summary
This summary is machine-generated.

SPATA2 is a newly identified molecule essential for regulating the Tumor Necrosis Factor Receptor 1 (TNFR1) signaling complex. Its absence leads to heightened inflammatory responses and impacts cell death pathways.

More Related Videos

Biochemical and Structural Characterization of the Carbohydrate Transport Substrate-binding-protein SP0092
08:53

Biochemical and Structural Characterization of the Carbohydrate Transport Substrate-binding-protein SP0092

Published on: October 2, 2017

31.8K
Efficient Sporulation of Saccharomyces cerevisiae in a 96 Multiwell Format
08:54

Efficient Sporulation of Saccharomyces cerevisiae in a 96 Multiwell Format

Published on: September 17, 2016

10.9K

Related Experiment Videos

Last Updated: Mar 6, 2026

Reconstitution of Msp1 Extraction Activity with Fully Purified Components
05:52

Reconstitution of Msp1 Extraction Activity with Fully Purified Components

Published on: August 10, 2021

3.0K
Biochemical and Structural Characterization of the Carbohydrate Transport Substrate-binding-protein SP0092
08:53

Biochemical and Structural Characterization of the Carbohydrate Transport Substrate-binding-protein SP0092

Published on: October 2, 2017

31.8K
Efficient Sporulation of Saccharomyces cerevisiae in a 96 Multiwell Format
08:54

Efficient Sporulation of Saccharomyces cerevisiae in a 96 Multiwell Format

Published on: September 17, 2016

10.9K

Area of Science:

  • Immunology
  • Molecular Biology
  • Cell Signaling

Background:

  • Tumor Necrosis Factor Receptor 1 (TNFR1) complex assembly relies on specific ubiquitylation patterns (K63- and M1-linked).
  • Deubiquitinases like CYLD and OTULIN dynamically regulate ubiquitylation processes.
  • The precise role of SPATA2 in modulating TNFR1 signaling was previously unclear.

Purpose of the Study:

  • To identify and characterize the function of the novel molecule SPATA2 in the TNFR1 signaling complex.
  • To investigate the impact of SPATA2 on ubiquitylation and deubiquitinase activity within the TNF-RSC.
  • To elucidate SPATA2's role in TNF- and NOD2-mediated inflammatory signaling and TNF-induced cell death.

Main Methods:

  • Identification of SPATA2 as a key component of the TNFR1 signaling complex.
  • Analysis of SPATA2's role in recruiting and activating the deubiquitinase CYLD.
  • Assessment of inflammatory signaling pathways (TNF- and NOD2) in the absence of SPATA2.
  • Evaluation of SPATA2's necessity for TNF-induced cell death.

Main Results:

  • SPATA2 is crucial for recruiting and activating CYLD within the TNF-RSC.
  • Loss of SPATA2 leads to elevated pro-inflammatory signaling via both TNF and NOD2 pathways.
  • SPATA2 is essential for mediating TNF-induced cell death.
  • Emerging evidence suggests SPATA2 may have functions beyond CYLD activation.

Conclusions:

  • SPATA2 is a critical regulator of the TNFR1 signaling complex, influencing both inflammatory responses and cell death.
  • The findings highlight SPATA2's multifaceted role in immune signaling.
  • Further research is warranted to fully understand SPATA2's biological functions.