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Analytical Methods for Metformin Estimation.
Durgawati Patel1, Puspendra Kumar2, Swapnil Sharma3
1a Department of Chemistry , Banasthali Vidyapith , Banasthali , India.
Metformin, a key drug for type II diabetes, can be accurately measured using various analytical techniques. This review covers methods like HPLC and LC-MS for analyzing metformin in medicines and biological samples.
Area of Science:
- Analytical Chemistry
- Pharmacology
- Biochemistry
Background:
- Metformin is a first-line oral anti-hyperglycemic medication for type II diabetes.
- Accurate quantification of metformin is crucial for therapeutic drug monitoring and quality control.
Purpose of the Study:
- To review and highlight diverse analytical methodologies for metformin estimation.
- To cover methods applicable to both pharmaceutical formulations and biological matrices.
Main Methods:
- High-Performance Liquid Chromatography (HPLC)
- High-Performance Thin-Layer Chromatography (HPTLC)
- Spectrophotometry
- Electrochemical methods
- Capillary Electrophoresis (CE)
- Liquid Chromatography-Mass Spectrometry (LC-MS)
Main Results:
- The article provides a comprehensive overview of established and emerging analytical techniques.
- Each method's applicability, sensitivity, and specificity for metformin determination are discussed.
Conclusions:
- A variety of validated analytical methods exist for accurate metformin quantification.
- The choice of method depends on the matrix, required sensitivity, and available resources.
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