Related Experiment Video
Updated: Mar 6, 2026
Studying Orthodontic Tooth Movement in Mice
Published on: August 2, 2024
Probiotic consumption decreases the number of osteoclasts during orthodontic movement in mice
Camila Alessandra Pazzini1, Luciano José Pereira2, Tarcília Aparecida da Silva3
1Department of Pediatric Dentistry and Orthodontics, Dental School, Universidade Federal de Minas Gerais - UFMG, Belo Horizonte, MG, Brazil.
Aims:
The aim of the present study was to investigate the effect of probiotic (Bacillus Subtilis) supplementation on bone remodelling induced by mechanical loading.
Methods:
C57BL/6 mice were divided in two groups: (1) Probiotic and (2) Vehicle (water). The probiotic (1.5×108CFU/mL) was administered orally for 14 days, starting two days before the induction of orthodontic tooth movement (OTM). OTM was determined by histomorphometric analysis by comparing the right to the left side of the maxilla. The number of osteoclasts was determined by counting TRAP-positive cells. Osteoblasts were counted on Masson's trichrome-stained slides.
Results:
OTM was similar between groups (with and without probiotic supplementation) (p=0.46). The number of TRAP-positive cells increased (p<0.01) on the experimental side (where the spring coil was installed) in comparison to the control side in both groups. However, the number of osteoclasts decreased (p˂0.01) in the probiotic group, in comparison to the vehicle group. There was an increase in the number of osteoblasts (p˂0.05) in both the Vehicle and Probiotic groups on the side under OTM, independent of probiotic supplementation.
Conclusion:
Oral Supplementation with a probiotic influenced the number of osteoclasts adjacent to the tooth root during orthodontic movement in mice.
