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In Vivo Microinjection and Electroporation of Mouse Testis
Published on: August 23, 2014
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Bacterial magnetic particles improve testes-mediated transgene efficiency in mice.
Chao Wang1, Guanghong Sun1, Ye Wang1
1a State Key Laboratories for Agrobiotechnology and College of Biological Sciences, China Agricultural University , Beijing , China.
Drug Delivery
|March 12, 2017
Summary
Nano-scaled bacterial magnetic particles (BMPs) complexed with polyethylenimine (PEI) offer an efficient method for gene transfer in mice. This novel approach significantly enhances the production of transgenic mice and improves sperm motility without causing testicular damage.
Area of Science:
- Biotechnology
- Gene Therapy
- Nanomaterials
Background:
- Nano-scaled materials are effective DNA carriers for transgenes.
- Bacterial magnetic particles (BMPs) can reduce the toxicity of polyethylenimine (PEI), a gene-transfer agent.
- BMPs assist in ex vivo tissue transgene delivery.
Purpose of the Study:
- To compare the effectiveness of BMP-PEI complex-conjugated foreign DNAs (BPDs) with liposome-conjugated foreign DNAs for testes-mediated gene transfer (TMGT) in mice.
- To evaluate the efficiency of BPDs in producing transgene founder mice and assess their impact on sperm motility and subsequent generations.
Main Methods:
- Testes injection of BPDs into mice.
- Comparison of BPDs with liposome-conjugated foreign DNAs.
- Assessment of transgene expression in testes and sperm motility.
- Evaluation of transgene transmission to the first filial generation.
Main Results:
- BPDs successfully reached the cytoplasm and nucleus of spermatogenesis cells in the testes.
- The ratio of founder mice obtained using BPDs (88%) was approximately three times higher than the control group (25%).
- Sperm motility in founder mice from the BPD group significantly improved compared to the control group.
- The ratio of transgene mice in the first filial generation was significantly higher with BPDs (73.8%) compared to the control (11.6%).
- No visible histological changes were observed in the testes after TMGT with BPDs.
Conclusions:
- Nano-scaled BPDs represent a viable alternative strategy for efficient in vivo production of transgene mice.
- BPDs enhance gene transfer efficiency and improve reproductive outcomes in transgenic mice.
- The BPD method is safe for testicular tissue, showing no adverse histological effects.
