Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Immunogold Electron Microscopy

Immunogold Electron Microscopy

Immunoelectron microscopy utilizes immunogold labeling of endogenous proteins with specific antibodies to detect and localize these proteins in cells and tissues. The procedure provides insights into the distribution and quantification of protein under different stimulation conditions offering clues about their functions. Conjugating highly electron-dense gold particles with primary or secondary antibodies allow antigen detection on and within cells, with high resolution and specificity.

Labeling DNA Probes

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...

Tagging and Fusion Proteins

Tagging and Fusion Proteins

Proteins are involved in several cellular processes and biochemical reactions. Analyzing a specific protein of interest requires it to be isolated from the other proteins in the cell. This is achieved by overexpressing the specific gene in a suitable host to produce large quantities of the target protein. A tag or label is recombined with the gene to produce a fusion protein containing the target protein and the tag. The tags on these fusion proteins can then be used for easy detection and...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

HDA19-mediated deacetylation of histone H3.3 at lysines 27 and 36 regulates plant sensitivity to salt stress.

Proceedings of the National Academy of Sciences of the United States of America·2026

Same author

(3R, 7S)-11-hydroxy-jasmonic acid is a major oxidative shunt product of jasmonic acid catabolism in Arabidopsis thaliana.

Nature communications·2026

Same author

Advances in the chemical biology of jasmonates.

Journal of experimental botany·2026

Same author

Bioorganic studies on the nyctinastic leaf-movement of plants.

Proceedings of the Japan Academy. Series B, Physical and biological sciences·2026

Same author

A Reactive Antagonist Strategy: Cysteine-Directed Covalent Molecular Glue in Plant Hormone Receptor Regulation.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026

Same author

Correction: Chemical application improves stress resilience in plants.

Plant molecular biology·2025

Search research articles

Related Experiment Video

Updated: Mar 6, 2026

Affinity Labeling Detection of Endogenous Receptors from Zebrafish Embryos

Affinity Labeling Detection of Endogenous Receptors from Zebrafish Embryos

Published on: August 31, 2016

Reactive group-embedded affinity labeling reagent for efficient intracellular protein labeling.

Yousuke Takaoka¹, Yuuki Nukadzuka², Minoru Ueda²

¹Department of Chemistry, Graduate School of Science, Tohoku University, 6-3 Aramaki-Aza-Aoba, Aoba-ku, Sendai 980-8578, Japan; Precursory Research for Embryonic Science and Technology PREST, Science and Technology Agency, JST, 4-1-8 Honcho, Kawaguchi, Saitama 332-0012, Japan.

Bioorganic & Medicinal Chemistry

|March 12, 2017

Summary

This study introduces a new strategy for labeling intracellular proteins in living cells using reactive group-embedded affinity labeling reagents. Ligand binding pocket-oriented reagents effectively label target proteins, unlike surface-oriented ones.

More Related Videos

Identification of Small Molecule-binding Proteins in a Native Cellular Environment by Live-cell Photoaffinity Labeling

Identification of Small Molecule-binding Proteins in a Native Cellular Environment by Live-cell Photoaffinity Labeling

Published on: September 20, 2016

Characterization of Neuronal Lysosome Interactome with Proximity Labeling Proteomics

Characterization of Neuronal Lysosome Interactome with Proximity Labeling Proteomics

Published on: June 23, 2022

Related Experiment Videos

Last Updated: Mar 6, 2026

Affinity Labeling Detection of Endogenous Receptors from Zebrafish Embryos

Affinity Labeling Detection of Endogenous Receptors from Zebrafish Embryos

Published on: August 31, 2016

Identification of Small Molecule-binding Proteins in a Native Cellular Environment by Live-cell Photoaffinity Labeling

Identification of Small Molecule-binding Proteins in a Native Cellular Environment by Live-cell Photoaffinity Labeling

Published on: September 20, 2016

Characterization of Neuronal Lysosome Interactome with Proximity Labeling Proteomics

Characterization of Neuronal Lysosome Interactome with Proximity Labeling Proteomics

Published on: June 23, 2022

Area of Science:

Chemical Biology
Molecular Biology
Biochemistry

Background:

Affinity labeling is crucial for chemical biology research.
Efficiently labeling intracellular proteins in living cells remains a challenge.

Purpose of the Study:

To develop a novel design strategy for reactive group-embedded affinity labeling reagents.
To improve the efficiency of intracellular protein labeling in living systems.

Main Methods:

Designed and synthesized affinity labeling reagents with embedded reactive groups.
Utilized FKBP12 as a model target protein for intracellular labeling experiments.
Investigated the efficacy of ligand binding pocket-oriented versus protein surface-oriented reagents.

Main Results:

A ligand binding pocket-oriented labeling reagent successfully labeled intracellular FKBP12 in living cells.
A protein surface-oriented reagent showed ineffectiveness for intracellular labeling.
Intracellular protein fluctuation under macromolecular crowding effects was identified as a contributing factor to labeling inefficiency.

Conclusions:

The proposed design strategy enables efficient intracellular protein labeling.
Pocket-oriented labeling reagents are superior for targeting intracellular proteins.
Understanding macromolecular crowding effects is key for optimizing labeling strategies.