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Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
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Proteins show rotational as well as lateral diffusion across the membrane. The lateral diffusion of proteins was confirmed through the cell fusion experiment where mouse and human cells were fused, resulting in hybrid cells. When the human and mouse cells fused, the specific membrane proteins on human and mouse cells were marked with the red and green-fluorescent markers, respectively. Initially, the red and green fluorescence was located on the respective hemisphere of the cell. As time...
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Dynamic cross-correlations between entangled biofilaments as they diffuse.

Boyce Tsang1, Zachary E Dell1, Lingxiang Jiang2

  • 1Department of Physics, University of Illinois at Urbana-Champaign, Urbana, IL 61801.

Proceedings of the National Academy of Sciences of the United States of America
|March 12, 2017
PubMed
Summary
This summary is machine-generated.

Entangled biofilaments exhibit long-range dynamic cross-correlations, exceeding hydrodynamic predictions in polymer physics. This emergent behavior arises from interpolymer repulsion in confined environments.

Keywords:
biofilamentcross-correlationentangledimagingreptation

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Area of Science:

  • Polymer Physics
  • Biological Physics
  • Soft Matter Physics

Background:

  • Entanglement in polymer physics describes how linear macromolecules diffuse anisotropically beyond a mesoscopic scale.
  • Macromolecules localize transversely but diffuse easily along their backbone, creating a broad spectrum of relaxation times.

Purpose of the Study:

  • To investigate the implications of entanglement in polymer and biological physics.
  • To experimentally demonstrate and theoretically model long-range dynamic cross-correlations in entangled biofilaments.

Main Methods:

  • Fluorescence tracking of dilutely labeled macromolecules (aqueous F-actin biofilaments).
  • Statistical mechanical modeling of the entangled system as rigid rods.
  • Analysis of dynamic cross-correlations and comparison with continuum hydrodynamics.

Main Results:

  • Entangled biofilaments showed separation-dependent dynamic cross-correlations extending up to ≈15 µm.
  • These correlations significantly exceeded predictions from continuum hydrodynamics.
  • A statistical mechanical theory based on de Gennes correlation hole successfully predicted these long-range correlations.

Conclusions:

  • Entanglement in biofilaments leads to emergent, long-range dynamic cross-correlations.
  • The de Gennes correlation hole mechanism explains these correlations, driven by chain connectivity and uncrossability.
  • The model assumes dynamically incompressible tubes for entangled biofilaments.