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A simplified plaque assay for varicella vaccine
J Husson-van Vliet1, G Colinet, F Yane
1Institut d' Hygiene et d' Epidemiologie, Departement Microbiologie, Brussels, Belgium.
Journal of Virological Methods
|November 1, 1987
Summary
A new plaque assay method offers a simple and accurate way to test the potency of attenuated varicella vaccines. This cost-effective assay provides reliable viral titre estimates for vaccine quality control.
Area of Science:
- Virology
- Vaccinology
- Cell Biology
Background:
- Potency testing is crucial for ensuring the efficacy and safety of vaccines.
- Attenuated varicella vaccine requires reliable methods for quality control.
Purpose of the Study:
- To describe a simple and accurate plaque assay for potency testing of attenuated varicella vaccine.
- To establish a cost-effective method for viral titre estimation.
Main Methods:
- Utilized the African green monkey kidney continuous cell line CV-1.
- Performed assays in multidish-plates under a semi-solid carboxymethylcellulose overlay.
- Assessed reliability through parallel titration of reference preparations.
Main Results:
- Developed a simple and accurate plaque assay method.
- The assay is economical and provides accurate individual titre estimates.
- Reliability of titre estimates can be confirmed using reference preparations.
Conclusions:
- The described plaque assay is suitable for routine potency testing of attenuated varicella vaccine.
- This method offers an economical and reliable approach to vaccine quality control.
- The assay's accuracy and reliability are validated by its performance with reference standards.