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Related Concept Videos

Protein Dynamics in Living Cells01:19

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Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
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DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
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Updated: Mar 6, 2026

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A fluorogenic probe for imaging protein S-nitrosylation in live cells.

Shiyi Shao1, Bo Chen2, Juan Cheng1

  • 1College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058, China.

Biosensors & Bioelectronics
|March 12, 2017
PubMed
Summary

Researchers developed a novel fluorogenic probe for detecting S-nitrosylation in live cells. This tool enables real-time imaging of S-nitrosylation dynamics, aiding research into vascular diseases.

Keywords:
Endothelial cellFluorescent probeImagingPosttranslational modificationS-nitrosylation

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Area of Science:

  • Biochemistry
  • Cell Biology
  • Chemical Biology

Background:

  • S-nitrosylation is a critical posttranslational modification impacting redox regulation and nitric oxide (NO) signaling.
  • Existing detection methods for S-nitrosylation are ex situ, limiting the study of its dynamic and reversible nature in live biological systems.

Purpose of the Study:

  • To develop and validate a fluorogenic chemical probe for detecting S-nitrosylation in intact live endothelial cells.
  • To enable real-time imaging of S-nitrosylation dynamics within live cellular environments.

Main Methods:

  • Development of a weakly emissive fluorogenic probe that becomes highly fluorescent upon reaction with S-nitrosothiols.
  • Application of the probe for in situ imaging of S-nitrosylation in live endothelial cells.
  • Validation of probe specificity and sensitivity by imaging glyceraldehyde-3-phosphate dehydrogenase (GAPDH) in live cells.

Main Results:

  • The developed probe exhibits high specificity and sensitivity for detecting S-nitrosylation in live endothelial cells.
  • The probe successfully visualized dynamic changes in protein S-nitrosylation in real-time.
  • The probe's utility was confirmed by imaging a known S-nitrosylation target, GAPDH, in live cells.

Conclusions:

  • The novel fluorogenic probe allows for effective in situ detection and imaging of S-nitrosylation in live cells.
  • This tool provides a promising platform for investigating the role of S-nitrosylation in the pathophysiology of vascular diseases.