Related Experiment Video
Updated: Mar 6, 2026

Improving Infrared Spectroscopy Characterization of Soil Organic Matter with Spectral Subtractions
Published on: January 10, 2019
Quantifying tropical peatland dissolved organic carbon (DOC) using UV-visible spectroscopy
Sarah Cook1, Mike Peacock2, Chris D Evans3
1Centre for Landscape & Climate Research, University of Leicester, Geography, Leicester, LE1 7RH, UK.
Abstract:
UV-visible spectroscopy has been shown to be a useful technique for determining dissolved organic carbon (DOC) concentrations. However, at present we are unaware of any studies in the literature that have investigated the suitability of this approach for tropical DOC water samples from any tropical peatlands, although some work has been performed in other tropical environments. We used water samples from two oil palm estates in Sarawak, Malaysia to: i) investigate the suitability of both single and two-wavelength proxies for tropical DOC determination; ii) develop a calibration dataset and set of parameters to calculate DOC concentrations indirectly; iii) provide tropical researchers with guidance on the best spectrophotometric approaches to use in future analyses of DOC. Both single and two-wavelength model approaches performed well with no one model significantly outperforming the other. The predictive ability of the models suggests that UV-visible spectroscopy is both a viable and low cost method for rapidly analyzing DOC in water samples immediately post-collection, which can be important when working at remote field sites with access to only basic laboratory facilities.
More Related Videos
Related Concept Videos
UV–Vis Spectrometers
Ultraviolet and Visible (UV–Vis) Spectroscopy: Overview
UV–Vis Spectroscopy: Beer–Lambert Law
UV–Vis Spectrum
The UV–Vis spectrum of a molecule is the plot of its absorbance versus wavelength. The plot is drawn by taking molar...
UV–Vis Spectroscopy: Woodward–Fieser Rules
UV–Vis Spectroscopy of Conjugated Systems
One of the factors influencing λmax is the extent of conjugation in...

