Physico-cultural parameters during AgNPs biotransformation with bactericidal activity against human pathogens

Area of Science:

• Nanotechnology
• Microbiology
• Biotechnology

Background:

• Developing efficient and cost-effective methods for silver nanoparticle (AgNP) synthesis is crucial.
• Cell extract-based synthesis offers a promising route for producing diverse nanoparticles.

Purpose of the Study:

• To systematically evaluate the impact of physico-cultural parameters on AgNP synthesis using P. plecoglossicida cell-free extract.
• To understand and control the morphology and surface characteristics of AgNPs.

Main Methods:

• Optimization of AgNP synthesis by varying growth media, precursor salt concentrations, pH, and temperature.
• Characterization of synthesized AgNPs for purity, morphology, and surface properties.
• Assessment of bactericidal activity against multi-drug resistant pathogens (A. baumannii, E. coli, P. aeruginosa, S. enterica) via disk diffusion tests.

Main Results:

• Physico-cultural parameters significantly influenced AgNP morphology, surface characteristics, production time, and yield.
• AgNPs exhibited varying levels of toxicity against tested pathogens.
• Higher toxicity was observed against E. coli and S. enterica compared to A. baumannii and P. aeruginosa.

Conclusions:

• The study successfully demonstrated control over AgNP characteristics through parameter optimization.
• The findings provide a foundation for scalable and efficient AgNP production for potential antimicrobial applications.