Polysaccharide from Mesona chinensis: Extraction optimization, physicochemical characterizations and antioxidant
Lihua Lin1, Jianhua Xie1, Suchen Liu1
1State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China.
Abstract:
The optimization of extraction conditions, physicochemical characterizations and antioxidant activities of polysaccharide from Mesona chinensis (MCP) were investigated. Optimal extraction conditions of MCP with the highest yield of 7.05±0.12% was obtained by response surface methodology (RSM). The physicochemical characterizations of MCP obtained at the optimal extraction conditions were detected by TU-1900 spectrophotometer, high performance gel permeation chromatography (HPGPC), high-performance anion exchange chromatography (HPAEC) and fourier transform infrared spectroscopy (FT-IR) as well, revealing that MCP was a heteropolysaccharide containing uronic acid (29.3±1.3%) and protein (10.4±0.8%), with an average molecular weight (Mw) of 1.45×106 Da, which mainly consisted of galactose (Gal) and glucose (Glc) in a molar ratio of 1.00:1.38. Furthermore, MCP exhibited considerable antioxidant potential on scavenging hydroxyl (53.87±0.44%), superoxide anion (58.42±1.17%) and DPPH radicals (55.59±0.69%). Our results indicated that MCP might be a good candidate for further investigations of functional foods.
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