Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Spindle Assembly02:50

Spindle Assembly

4.4K
Spindle assembly occurs through three, often coexisting, pathways – the centrosome-mediated pathway, the chromatin-mediated pathway, and the microtubule-mediated pathway – collectively contributing to form a robust spindle apparatus.
In most cells, centrosomes are the primary microtubule nucleation centers. In the centrosome-mediated pathway, the G2-prophase transition triggers centrosome maturation and increased microtubule nucleation. Progressive nucleation results in a...
4.4K
The Spindle Assembly Checkpoint02:19

The Spindle Assembly Checkpoint

3.9K
The spindle assembly checkpoint is a molecular surveillance mechanism ensuring the fidelity of chromosome segregation during anaphase. The checkpoint monitors the completion of all the prerequisite steps before chromosome segregation to determine whether the segregation process should proceed or be delayed.
Many proteins function together to control the spindle assembly checkpoint. Mutations affecting these proteins may allow cells to proceed into anaphase prematurely, resulting in the...
3.9K
Determining the Plane of Cell Division02:13

Determining the Plane of Cell Division

4.0K
Positioning the cell division plane is a critical step during development and cell differentiation, particularly during mitosis when the plane is essential for determining the size of the two daughter cells. The cell division plane is perpendicular to the plane of chromosome segregation, but different types of organisms have different cell division mechanisms to suit their morphology and function. 
Animal cells
In animal cells, the cleavage furrow forms along the plane of cell division...
4.0K
The Mitotic Spindle02:27

The Mitotic Spindle

8.2K
The mitotic spindle—or spindle apparatus—is a eukaryotic, cytoskeletal structure made up of long protein fibers called microtubules. Formed during cell division, the spindle separates sister chromatids and moves them to opposite ends of a parental cell, where the now individual chromosomes are distributed to two daughter cell nuclei.
The bipolar configuration of the mitotic spindle facilitates chromosomal segregation, preparing the cell for division. One mechanism that ensures...
8.2K
Spermatogenesis01:41

Spermatogenesis

124.2K
Spermatogenesis is the process by which haploid sperm cells are produced in the male testes. It starts with stem cells located close to the outer rim of seminiferous tubules. These spermatogonial stem cells divide asymmetrically to give rise to additional stem cells (meaning that these structures “self-renew”), as well as sperm progenitors, called spermatocytes. Importantly, this method of asymmetric mitotic division maintains a population of spermatogonial stem cells in the male...
124.2K
Attachment of Sister Chromatids02:57

Attachment of Sister Chromatids

4.1K
As cells progress into mitosis, the nuclear envelope breaks down, and the condensed chromosomes are exposed to the array of bipolar microtubules of the mitotic spindle. The kinetochore, a large, disc-shaped protein complex, is present at the centromere region of the sister chromatids and acts as a binding site for the microtubules.  Usually, the plus-end of a single microtubule is embedded within the kinetochore. However, some kinetochores first establish lateral contact with the side-wall...
4.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

SMARCA4 loss reprograms p300 chromatin occupancy to subvert p53-mediated transcriptional repression in ovarian small cell carcinoma.

Nature communications·2026
Same author

Associations Between Anthropometrics, Physical Determinants, and Batting Exit Speed in Elite Female Baseball Athletes.

Journal of strength and conditioning research·2026
Same author

Distinct immune landscapes characterize highly versus minimally invasive brain metastases.

JCI insight·2026
Same author

ATR and PKMYT1 Inhibition Resensitizes a Subset of TNBC Patient-Derived Models to Carboplatin, Inducing Mitotic Catastrophe.

Cancer research communications·2026
Same author

Apoptotic cell clearance triggers epithelial fate reprogramming during prostate regression.

Cell death & disease·2026
Same author

SMARCA4/2 loss reduces BCL-xL expression and confers a druggable MCL1 dependency in cancer.

NPJ precision oncology·2026

Related Experiment Video

Updated: Mar 6, 2026

Evaluating the Differentiation Capacity of Mouse Prostate Epithelial Cells Using Organoid Culture
10:38

Evaluating the Differentiation Capacity of Mouse Prostate Epithelial Cells Using Organoid Culture

Published on: November 22, 2019

9.6K

Lineage Specification from Prostate Progenitor Cells Requires Gata3-Dependent Mitotic Spindle Orientation.

Maxwell E R Shafer1, Alana H T Nguyen2, Mathieu Tremblay1

  • 1Rosalind and Morris Goodman Cancer Research Centre, McGill University, 1160 Pine Avenue West, Room 415, Montreal, QC H3A 1A3, Canada; Department of Biochemistry, McGill University, 3655 Promenade Sir William Osler, Montreal, QC H3G 1Y6, Canada.

Stem Cell Reports
|March 14, 2017
PubMed
Summary
This summary is machine-generated.

GATA3 regulates prostate development by controlling cell division orientation. Loss of GATA3 disrupts spindle orientation, leading to abnormal cell lineage expansion and tissue defects.

Keywords:
GATA3atypical protein kinase Caurothiomalatecell polarityepithelial stratificationlineage specificationpar complexprostate developmentprostate progenitor cellsspindle orientation

More Related Videos

Isolation of Stem-like Cells from 3-Dimensional Spheroid Cultures
09:06

Isolation of Stem-like Cells from 3-Dimensional Spheroid Cultures

Published on: December 13, 2019

9.6K
Live Cell Imaging to Assess the Dynamics of Metaphase Timing and Cell Fate Following Mitotic Spindle Perturbations
07:14

Live Cell Imaging to Assess the Dynamics of Metaphase Timing and Cell Fate Following Mitotic Spindle Perturbations

Published on: September 20, 2019

8.8K

Related Experiment Videos

Last Updated: Mar 6, 2026

Evaluating the Differentiation Capacity of Mouse Prostate Epithelial Cells Using Organoid Culture
10:38

Evaluating the Differentiation Capacity of Mouse Prostate Epithelial Cells Using Organoid Culture

Published on: November 22, 2019

9.6K
Isolation of Stem-like Cells from 3-Dimensional Spheroid Cultures
09:06

Isolation of Stem-like Cells from 3-Dimensional Spheroid Cultures

Published on: December 13, 2019

9.6K
Live Cell Imaging to Assess the Dynamics of Metaphase Timing and Cell Fate Following Mitotic Spindle Perturbations
07:14

Live Cell Imaging to Assess the Dynamics of Metaphase Timing and Cell Fate Following Mitotic Spindle Perturbations

Published on: September 20, 2019

8.8K

Area of Science:

  • Cell biology
  • Developmental biology
  • Prostate cancer research

Background:

  • Prostate development involves basal and luminal cell generation from bipotent basal cells.
  • Spindle orientation's role in division symmetry and cell fate during prostate development is not fully understood.
  • Upstream regulators of spindle orientation in prostate progenitors remain elusive.

Purpose of the Study:

  • To investigate the role of GATA3 in prostate progenitor cell division and lineage specification.
  • To identify upstream factors regulating mitotic spindle orientation in the developing prostate.
  • To elucidate the mechanism by which GATA3 influences cell fate and tissue organization.

Main Methods:

  • GATA3 expression analysis in prostate progenitor and luminal cells.
  • Investigating the effects of GATA3 loss on PRKCZ localization and mitotic spindle orientation.
  • Analyzing cell lineage dynamics and tissue morphology following GATA3 manipulation.
  • Examining the impact of disrupting PRKCZ and PARD6B interactions on cell phenotypes.

Main Results:

  • GATA3 is expressed in prostate basal progenitor and luminal cells.
  • Loss of GATA3 causes PRKCZ mislocalization, leading to spindle randomization in progenitor divisions.
  • Accumulation of proliferative intermediate progenitor cells and expansion of the luminal compartment observed.
  • Disruption of PRKCZ-PARD6B interaction phenocopies spindle and cell lineage defects.

Conclusions:

  • GATA3 plays a critical role in prostate lineage specification by regulating spindle orientation.
  • Proper spindle orientation is essential for maintaining hierarchical cell lineage organization in the prostate.
  • The GATA3-PRKCZ-PARD6B axis is crucial for controlling cell division asymmetry and prostate tissue development.