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Role of thymic B cells in the development of thymus-derived regulatory T cell in vitro

Haider H Mohammed Ali1, Nadzieja Drela2

  • 1University of Warsaw, Faculty of Biology, Department of Immunology, Miecznikowa 1, 02-096 Warsaw, Poland; Department of Biology, College of Science, University of Kerbala, Kerbala, Iraq.

Immunology Letters
|March 14, 2017
PubMed

The thymus contains a low frequency of B cells, about 0.1-0.5% of total population of thymocytes that were shown to contribute in thymic negative selection. The fact that B cells in the periphery have been contributed in the generation of the T regulatory cell compartment emerged the idea that this process may indeed be initiated firstly within the thymus. The results of this study revealed that activated thymic B cells maintained the high percentage of nTreg generation or counteracted the decrease of this percentage observed in non-activated culture, and both activators (LPS and IMQ) have the same effect in the process of nTreg generation by B cells. In addition, the activated cultures showed increase of the level of expression of Foxp3 transcription factor. In this study we confirmed that thymic B cells in the condition of our experiments did not influence the generation of nTregs, but rather maintain their viability when activated by both activators.

Keywords:
Costimulatory moleculesIMQLPSMHC IIThymic B cellsnTregs

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