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Variation of R1 repeated sequence present in open reading frame 11 of varicella-zoster virus strains
H Kinoshita1, R Hondo, F Taguchi
1Department of Microbiology, School of Hygienic Sciences, Kitasato University, Kanagawa, Japan.
Abstract:
We molecularly cloned the tandem direct reiteration (R1) present in open reading frame (ORF) 11 from three independent strains of varicella-zoster virus. Comparison of the R1 sequences among varicella-zoster virus strains revealed that, although the portion of R1 near the 5' terminus of ORF 11 was conserved among strains, the 3'-terminal portion varied remarkably. This variation was due to the different arrangement of two elements (A and B) and a segment produced by fusion of A and B and to a single-base change in the A element. Since the difference in the size of R1 among strains was a multiple of 3 base pairs, the variation in R1 caused no frame shift in ORF 11.