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Assessment of de novo Protein Synthesis Rates in Caenorhabditis elegans
Published on: September 12, 2020
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Protein Synthesis Rate Assessment by Fluorescence Recovery after Photobleaching (FRAP)
Nikos Kourtis1, Nektarios Tavernarakis2
1Institute of Molecular Biology and Biotechnology, Foundation for Research and Technology Hellas, Crete, Greece; Department of Pathology, NYU School of Medicine, New York, USA.
Bio-Protocol
|March 14, 2017
Abstract:
Currently available biochemical methods cannot be applied to monitor protein synthesis in specific cells or tissues, in live specimens. Here, we describe a non-invasive method for monitoring protein synthesis in single cells or tissues with intrinsically different translation rates, in live Caenorhabditis elegans animals.

