Fluorogenic Peptide Substrate for Quantification of Bacterial Enzyme Activities

A novel peptide substrate (A G G P L G P P G P G G) was developed for quantifying the activities of bacterial enzymes using a highly sensitive Fluorescence Resonance Energy Transfer (FRET) based assay. The peptide substrate was cleaved by collagenase class I, II, Liberase MTF C/T, collagenase NB1, and thermolysin/neutral protease, which was significantly enhanced in the presence of CaCl₂. However, the activities of these enzymes were significantly decreased in the presence of ZnSO₄ or ZnCl₂. Collagenase I, II, Liberase MTF C/T, thermolysin/neutral protease share similar cleavage sites, L^↓G and P^↓G. However, collagenase NB1 cleaves the peptide substrate at G^↓P and P^↓L, in addition to P^↓G. The enzyme activity is pH dependent, within a range of 6.8 to 7.5, but was significantly diminished at pH 8.0. Interestingly, the peptide substrate was not cleaved by endogenous pancreatic protease such as trypsin, chymotrypsin, and elastase. In conclusion, the novel peptide substrate is collagenase, thermolysin/neutral protease specific and can be applied to quantify enzyme activities from different microbes. Furthermore, the assay can be used for fine-tuning reaction mixtures of various agents to enhance the overall activity of a cocktail of multiple enzymes and achieve optimal organ/tissue digestion, while protecting the integrity of the target cells.