Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Mar 6, 2026

Temporal Ordering of Dynamic Expression Data from Detailed Spatial Expression Maps
11:52

Temporal Ordering of Dynamic Expression Data from Detailed Spatial Expression Maps

Published on: February 9, 2017

6.6K

Temporal Ordering of Dynamic Expression Data from Detailed Spatial Expression Maps.

Charlotte S L Bailey1, Robert A Bone1, Philip J Murray2

  • 1The Danish Stem Cell Center (DanStem), University of Copenhagen.

Journal of Visualized Experiments : Jove
|March 14, 2017
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A toolkit for mapping cell identities in relation to neighbors reveals conserved patterning of neuromesodermal progenitor populations.

PLoS biology·2025
Same author

NOTCH1 S2513 is critical for the regulation of NICD levels impacting the segmentation clock in hiPSC-derived PSM cells and somitoids.

Genes & development·2025
Same author

Altering metabolism programs cell identity via NAD<sup>+</sup>-dependent deacetylation.

The EMBO journal·2025
Same author

LiverZap: a chemoptogenetic tool for global and locally restricted hepatocyte ablation to study cellular behaviours in liver regeneration.

Development (Cambridge, England)·2024
Same author

Space and time on the membrane: modelling Type VI secretion system dynamics as a state-dependent random walk.

Royal Society open science·2023
Same author

Autoregulation of Transcription and Translation: A Qualitative Analysis.

Bulletin of mathematical biology·2023
Same journal

A Video Protocol of a Randomized Controlled Clinical Trial - Electrochemotherapy of Cutaneous Metastases with Reduced Dose Bleomycin (BLESS Trial).

Journal of visualized experiments : JoVE·2026
Same journal

A Standardized Ex Vivo Porcine Oromucosal Model for Evaluating Peptide Fluxes.

Journal of visualized experiments : JoVE·2026
Same journal

Lightweight English Text Classification with Deep Learning Based on Complex System Theory.

Journal of visualized experiments : JoVE·2026
Same journal

Integrating Artificial Intelligence-Assisted Translation Support into English Courses: Effects on Translation Accuracy, Perceived Stress, and Anxiety.

Journal of visualized experiments : JoVE·2026
Same journal

A Toxin-Based Counter-Selection System for Markerless Gene Deletion and High-Density Tn5 Transposon Mutagenesis in Pectobacterium brasiliense.

Journal of visualized experiments : JoVE·2026
Same journal

Seamless Multimodal Human-Robot Communication: Integration Techniques in Human-Computer Interaction.

Journal of visualized experiments : JoVE·2026
See all related articles

The segmentation clock regulates somitogenesis periodicity via oscillating gene expression. This study visualizes Notch signaling dynamics, revealing how receptor and ligand expression patterns contribute to synchronized cell oscillations during development.

Area of Science:

  • Developmental Biology
  • Molecular Biology
  • Cell Biology

Background:

  • Somitogenesis involves periodic formation of epithelial somites regulated by a molecular segmentation clock.
  • Clock genes, including Wnt, Notch, and FGF signaling pathway components, drive oscillatory gene expression in the presomitic mesoderm (PSM).
  • Notch signaling synchronizes oscillations between neighboring cells, with its intracellular domain (NICD) exhibiting oscillatory production and degradation.

Purpose of the Study:

  • To investigate the spatio-temporal dynamics of Notch signaling components during somitogenesis.
  • To visualize the dynamic expression profiles of Notch receptor, ligand, and target clock genes throughout an oscillation cycle.
  • To understand how dynamic Notch receptor and Delta ligand expression contributes to intercellular coupling and oscillation synchronization.

More Related Videos

Mining Spatial Transcriptomics Datasets using DeepSpaceDB
10:16

Mining Spatial Transcriptomics Datasets using DeepSpaceDB

Published on: September 5, 2025

920
Comprehensive Spatial Profiling of Species-agnostic Transcriptomes via Stereo-seq
10:22

Comprehensive Spatial Profiling of Species-agnostic Transcriptomes via Stereo-seq

Published on: October 31, 2025

686

Related Experiment Videos

Last Updated: Mar 6, 2026

Temporal Ordering of Dynamic Expression Data from Detailed Spatial Expression Maps
11:52

Temporal Ordering of Dynamic Expression Data from Detailed Spatial Expression Maps

Published on: February 9, 2017

6.6K
Mining Spatial Transcriptomics Datasets using DeepSpaceDB
10:16

Mining Spatial Transcriptomics Datasets using DeepSpaceDB

Published on: September 5, 2025

920
Comprehensive Spatial Profiling of Species-agnostic Transcriptomes via Stereo-seq
10:22

Comprehensive Spatial Profiling of Species-agnostic Transcriptomes via Stereo-seq

Published on: October 31, 2025

686

Main Methods:

  • Culture of presomitic mesoderm (PSM) explants.
  • Sensitive detection methods for mRNA and protein staining.
  • Confocal image analysis and computational modeling for temporal ordering of expression data.
  • Generation of kymographs to visualize spatio-temporal expression patterns.

Main Results:

  • Dynamic expression patterns of Notch receptor (Notch1) and Delta ligand (Dll1) at both mRNA and protein levels were observed.
  • Spatio-temporal expression profiles of Dll1, Notch1, and Notch target clock genes were constructed throughout an oscillation cycle.
  • Computational analysis enabled the visualization of these dynamic expression patterns, creating kymographs.

Conclusions:

  • The study provides a detailed spatio-temporal map of Notch signaling dynamics during somitogenesis.
  • Dynamic expression of Notch receptor and ligand is crucial for intercellular coupling and synchronizing segmentation clock oscillations.
  • These findings enhance our understanding of the molecular mechanisms underlying developmental periodicity.