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Related Experiment Video

Updated: Mar 6, 2026

Experimental Protocol for Detecting Cyanobacteria in Liquid and Solid Samples with an Antibody Microarray Chip
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Experimental Protocol for Detecting Cyanobacteria in Liquid and Solid Samples with an Antibody Microarray Chip.

Yolanda Blanco1, Mercedes Moreno-Paz1, Victor Parro2

  • 1Department of Molecular Evolution, Centro de Astrobiología (CAB, INTA-CSIC).

Journal of Visualized Experiments : Jove
|March 14, 2017
PubMed
Summary

Rapid detection of toxin-producing cyanobacteria is crucial for water safety. A new antibody microarray chip (CYANOCHIP) enables quick, in-field identification of harmful algal bloom strains.

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Area of Science:

  • Environmental Science
  • Microbiology
  • Analytical Chemistry

Background:

  • Global warming and eutrophication drive harmful cyanobacterial blooms in aquatic ecosystems.
  • Toxin-producing cyanobacteria pose significant health and economic risks.
  • Effective early-warning systems are needed for water resource management.

Purpose of the Study:

  • To develop and validate an in-field method for detecting toxin-producing cyanobacterial strains.
  • To provide a rapid and reliable tool for water authorities.

Main Methods:

  • Utilized a multiplex fluorescent sandwich microarray immunoassay (FSMI) with a CYANOCHIP containing 17 antibodies.
  • Developed protocols for analyzing both liquid and solid (sediment) water samples.
  • Employed a portable fluorescence detector for rapid results.

Main Results:

  • The CYANOCHIP allows simultaneous monitoring of 17 common freshwater cyanobacterial strains.
  • The entire detection process, including incubation, takes approximately 3 hours.
  • The assay provides visual detection of cyanobacterial markers via fluorescence.

Conclusions:

  • The CYANOCHIP offers a rapid, in-field solution for identifying toxin-producing cyanobacteria.
  • This technology supports timely decision-making for managing harmful algal blooms.
  • The method is adaptable for various water sample types, enhancing its practical application.