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Published on: August 23, 2019
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Microarray profiling of circular RNAs in human papillary thyroid carcinoma.
Nianchun Peng1, Lixin Shi1, Qiao Zhang1
1Department of Endocrinology and Metabolism, Affiliated Hospital of Guizhou Medical University, Guiyang, China.
Plos One
|March 14, 2017
Summary
Circular RNAs (circRNAs) are dysregulated in papillary thyroid carcinoma (PTC). This study identified key circRNAs, including hsa_circRNA_100395, and their interactions with miRNAs, suggesting their role in PTC pathogenesis and potential as biomarkers.
Area of Science:
- Oncology
- Molecular Biology
- Genomics
Background:
- Non-coding circular RNAs (circRNAs) exhibit altered expression in various human cancers.
- Understanding circRNA expression in papillary thyroid carcinoma (PTC) is crucial for elucidating its pathogenesis.
Purpose of the Study:
- To profile circRNA expression in PTC tumors.
- To identify differentially expressed circRNAs in PTC.
- To investigate potential circRNA-miRNA interactions and their role in PTC.
Main Methods:
- Microarray profiling of 18 thyroid tissue samples (PTC, normal, benign).
- Hierarchical clustering and statistical analysis to identify differential circRNAs.
- qRT-PCR validation, miRNA target prediction, and network construction for circRNA-miRNA interactions.
Main Results:
- Identified 88 upregulated and 10 downregulated circRNAs in PTC vs. normal tissue.
- Found 129 upregulated and 226 downregulated circRNAs in PTC vs. benign lesions.
- Discovered hsa_circRNA_100395 downregulation and its interaction with miR-141-3p and miR-200a-3p, potentially targeting cancer-related genes.
Conclusions:
- circRNA dysregulation is implicated in PTC pathogenesis.
- Specific circRNAs, like hsa_circRNA_100395, show potential as PTC biomarkers.
- The hsa_circRNA_100395/miR-141-3p/miR-200a-3p axis may play a significant role in PTC development.
