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CRISPR

CRISPR

Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...

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Related Experiment Video

Updated: Mar 6, 2026

Dissection of Enhancer Function Using Multiplex CRISPR-based Enhancer Interference in Cell Lines

Dissection of Enhancer Function Using Multiplex CRISPR-based Enhancer Interference in Cell Lines

Published on: June 2, 2018

Guide Picker is a comprehensive design tool for visualizing and selecting guides for CRISPR experiments.

Soren H Hough¹, Kris Kancleris², Leigh Brody²

¹Desktop Genetics, Ltd., 28 Hanbury Street, London, E1 6QR, UK. sorenh@desktopgenetics.com.

BMC Bioinformatics

|March 15, 2017

Summary

Guide Picker is a web tool that simplifies CRISPR experimental design by offering ten guide RNA scoring functions in one interface. It enables rapid selection of optimal guides for gene editing in human and mouse models.

Keywords:

CRISPR Cas9 Functional genomics Genome editing RNA guide design software sgRNA

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Last Updated: Mar 6, 2026

Dissection of Enhancer Function Using Multiplex CRISPR-based Enhancer Interference in Cell Lines

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Published on: June 2, 2018

Investigation of Genetic Dependencies Using CRISPR-Cas9-based Competition Assays

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CIRCLE-Seq for Interrogation of Off-Target Gene Editing

CIRCLE-Seq for Interrogation of Off-Target Gene Editing

Published on: November 1, 2024

Area of Science:

Molecular Biology
Genomics
Bioinformatics

Background:

CRISPR-Cas9 gene editing requires careful selection of guide RNAs (gRNAs) for experimental success.
Existing tools often present limited options for gRNA scoring and visualization.
A meta-tool is needed to consolidate and simplify gRNA design for CRISPR experiments.

Purpose of the Study:

To develop and present Guide Picker, a novel online tool for comprehensive CRISPR guide RNA design.
To integrate ten different gRNA scoring algorithms into a single, user-friendly interface.
To facilitate efficient identification and selection of optimal gRNAs for CRISPR applications.

Main Methods:

Guide Picker utilizes a multidimensional graphical display with two plots and four axes.
The tool analyzes all potential gRNAs targeting protein-coding regions in human and mouse genes.
Pre-computed scores for all gRNAs are used for rapid analysis and filtering based on multiple criteria.

Main Results:

Guide Picker allows simultaneous visualization and sorting of numerous gRNAs.
Investigators can filter gRNAs based on up to four distinct criteria.
The tool provides rapid generation and selection of high-scoring guide RNAs.

Conclusions:

Guide Picker enhances the CRISPR experimental design process through its intuitive interface.
The tool's flexibility supports diverse genome editing applications.
Its ease-of-use complements the power and modularity of CRISPR technology.