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Related Concept Videos

CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

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The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
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CRISPR01:59

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Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
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CRISPR and crRNAs02:53

CRISPR and crRNAs

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Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
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Autophagy01:27

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Autophagy is a self-digesting process by which a cell protects itself from threats both within and outside the cell, ranging from abnormal proteins to invading bacteria. In this process, obsolete components of the cell and invading microbes are degraded by hydrolytic enzymes active in an acidic environment of the lysosomal lumen.
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Homologous Recombination02:31

Homologous Recombination

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The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
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The Antiviral System of Bacteria and Archaea: CRISPR01:23

The Antiviral System of Bacteria and Archaea: CRISPR

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CRISPR stands for Clustered Regularly Interspaced Short Palindromic Repeats is a adaptive immune system found in bacteria and archaea that protects against viral infections. This system enables prokaryotic cells to identify, remember, and neutralize foreign genetic elements, primarily bacteriophages, by storing fragments of the invader’s DNA as a genetic memory.The CRISPR immune response begins during an initial infection. Cas (CRISPR-associated) proteins play a central role in this...
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Genome Editing in Mammalian Cell Lines using CRISPR-Cas
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Genome Editing in Mammalian Cell Lines using CRISPR-Cas

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CRISPR system for genome engineering: the application for autophagy study.

Jianzhou Cui1, Shirley Jia Li Chew1, Yin Shi1

  • 1Department of Physiology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore.

BMB Reports
|March 15, 2017
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Summary
This summary is machine-generated.

The CRISPR/Cas9 system offers precise genome editing, surpassing older tools like ZFNs and TALENs. This review details CRISPR/Cas9

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A Standard Methodology to Examine On-site Mutagenicity As a Function of Point Mutation Repair Catalyzed by CRISPR/Cas9 and SsODN in Human Cells
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Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms
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Related Experiment Videos

Last Updated: Mar 6, 2026

Genome Editing in Mammalian Cell Lines using CRISPR-Cas
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A Standard Methodology to Examine On-site Mutagenicity As a Function of Point Mutation Repair Catalyzed by CRISPR/Cas9 and SsODN in Human Cells
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Area of Science:

  • Molecular Biology
  • Genetics
  • Biotechnology

Background:

  • CRISPR/Cas9 is a revolutionary genome engineering tool.
  • It offers advantages over previous technologies like meganucleases, zinc finger nucleases (ZFNs), and transcription activator-like effectors (TALENs).
  • Its simplicity and efficiency enable multiplexed genome targeting in various species.

Purpose of the Study:

  • To review the history, types, structure, and mechanism of action of the CRISPR/Cas system.
  • To highlight the application of CRISPR/Cas9 in autophagy research.

Main Methods:

  • Literature review of CRISPR/Cas9 technology.
  • Analysis of CRISPR/Cas9 applications in biological research, specifically autophagy.

Main Results:

  • CRISPR/Cas9 system is a highly efficient and versatile genome editing tool.
  • The system's design facilitates ease of use and high-efficiency editing through guide RNAs.
  • It has expanded possibilities for genetic perturbation in previously intractable species.

Conclusions:

  • CRISPR/Cas9 is revolutionizing biological research, medicine, and biotechnology.
  • The system's application in autophagy research demonstrates its potential for advancing scientific understanding.
  • CRISPR/Cas9 paves a bright future for genetic engineering and therapeutic development.