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Isolation, Culture, and Characterization of Primary Dermal Fibroblasts from Human Keloid Tissue
Published on: July 28, 2023
Suppression of transforming growth factor-beta1 expression in keloids after cryosurgery
Sara M Awad1, Sahar A Ismail1, Doaa S Sayed1
1Department of Dermatology, Faculty of Medicine, Assiut University, Assiut, Egypt.
Abstract:
The biological mechanism underlying cryosurgical treatment of keloids remains unclear. Transforming growth factor-beta1 (TGF-β1) has been implicated in the pathogenesis of keloids and was reported to be the target of several therapeutic modalities. However, the effect of cryosurgery on its expression in keloid tissue has not been yet investigated. In this study, 26 consecutive keloid patients were treated with cryosurgery for 2-6 sessions. Keloids were biopsied before starting cryosurgery and after two treatment sessions for the immunohistochemical evaluation of TGF-β1 expression. The average volume reduction, after two treatment sessions (in 22 patients completing the study) was 68.77 ± 15.82%. Dermal overexpression of TGF-β1 was demonstrated in all keloid specimens before treatment. Following therapy, significant reduction of that expression was detected in all keloid specimens (P = 0.016). In addition to attesting the clinical efficacy of cryosurgery, our findings indicate that cryosurgery effectively suppressed TGF-β1 expression, possibly contributing to keloid regression.
Insights
Cryosurgery effectively reduces keloid volume and suppresses dermal overexpression of transforming growth factor-beta1 (TGF-β1), a key factor in keloid development.
Area of Science:
- Dermatology
- Oncology
- Immunology
Background:
- The biological mechanisms of cryosurgical treatment for keloids are not fully understood.
- Transforming growth factor-beta1 (TGF-β1) is implicated in keloid pathogenesis and targeted by various therapies.
- The impact of cryosurgery on TGF-β1 expression in keloid tissue has not been previously investigated.
Purpose of the Study:
- To investigate the effect of cryosurgery on TGF-β1 expression in keloid tissue.
- To evaluate the clinical efficacy of cryosurgery in keloid treatment.
Main Methods:
- 26 keloid patients underwent 2-6 cryosurgery sessions.
- Keloid biopsies were collected before treatment and after two sessions.
- Immunohistochemical evaluation of TGF-β1 expression was performed.
Main Results:
- An average keloid volume reduction of 68.77% was observed after two sessions.
- Dermal TGF-β1 overexpression was present in all pre-treatment keloid specimens.
- Significant reduction in TGF-β1 expression was detected post-cryosurgery (P=0.016).
Conclusions:
- Cryosurgery demonstrates clinical efficacy in treating keloids.
- Cryosurgery effectively suppresses TGF-β1 expression in keloid tissue.
- Reduced TGF-β1 expression may contribute to keloid regression following cryosurgery.
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