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Sample Preparation for Mass Spectrometry-based Identification of RNA-binding Regions
Published on: September 28, 2017
Analyses of mRNA Profiling through RNA Sequencing on a SAMP8 Mouse Model in Response to Ginsenoside Rg1 and Rb1
Shuai Zhang1, Dina Zhu1, Hong Li1
1Beijing Area Major Laboratory of Protection and Utilization of Traditional Chinese Medicine, Beijing Normal UniversityBeijing, China; Engineering Research Center of Natural Medicine, Ministry of Education, Beijing Normal UniversityBeijing, China; Department of Chinese Medicine, College of Resources Science Technology, Beijing Normal UniversityBeijing, China.
Ginsenoside Rg1 and Rb1 may help combat Alzheimer's disease (AD) by improving memory in mice. This study reveals their genome-level mechanisms, offering a basis for new anti-AD drug development.
Area of Science:
- Neuroscience
- Pharmacology
- Genomics
Background:
- Ginsenoside Rg1 (GRg1) and Ginsenoside Rb1 (GRb1) are key components in traditional Chinese medicines.
- These compounds are recognized for their potential to mitigate cognitive decline and protect against Alzheimer's disease (AD).
- Previous research and preliminary experiments suggest GRg1 and GRb1 can reduce memory deficits in senescence-accelerated prone 8 (SAMP8) mice.
Purpose of the Study:
- To systematically investigate the anti-AD mechanisms of GRg1 and GRb1 at the genome level.
- To elucidate the effects of these ginsenosides on gene expression in the brains of SAMP8 mice using deep RNA sequencing.
- To provide a gene-level explanation for the efficacy of GRg1 and GRb1 in an AD mouse model.
Main Methods:
- Utilized a senescence-accelerated mouse prone 8 (SAMP8) model to simulate AD progression.
- Performed deep RNA sequencing to analyze genome-wide mRNA transcript expression.
- Analyzed differentially expressed genes using gene ontology and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis.
Main Results:
- Identified 1,780 differentially expressed mRNA transcripts between SAMP8 and SAMP8+GRg1 mice.
- Detected 1,066 significantly dysregulated mRNA transcripts between SAMP8 and SAMP8+GRb1 mice.
- Found that GRg1 and GRb1 administration improved learning in SAMP8 mice, impacting pathways like nervous system development and MAPK signaling.
Conclusions:
- GRg1 and GRb1 demonstrate significant therapeutic potential against Alzheimer's disease at the genetic level.
- The study provides the first systematic mRNA profiling of GRg1 and GRb1 treatment in SAMP8 mouse brains.
- Findings support the development of GRg1 and GRb1 as functional drugs for Alzheimer's disease treatment.
