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Anti-idiotypic antibodies raised against anti-metenkephalin antibodies in rabbits

M F Jen1, Y X Liu, Y C Chin

  • 1Department of Pharmacology, Chinese Academy of Medical Sciences, Beijing, China.

NIDA Research Monograph
|January 1, 1986
PubMed

Anti-idiotypic antibodies were raised in rabbits immunized against immunoglobulins purified from anti-metenkephalin antibody. The antibodies competed dose-dependently with metenkephalin in binding to anti-metenkephalin antibody on a solid phase metenkephalin enzyme-linked immunosorbent assay (ELISA). The titers of the antiidiotypic activity appeared characteristically in transient peaks and troughs in alternation with anti-metenkephalin activities and were retained after purification on various affinity columns.

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