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Receptor Autoradiography Protocol for the Localized Visualization of Angiotensin II Receptors
12:03

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Published on: June 7, 2016

Autoradiographic localization of opioid receptor types in the rat small intestine

M R Dashwood1, R M Sykes, C S Thompson

  • 1Department of Physiology, Royal Free Hospital School of Medicine, London, England.

NIDA Research Monograph
|January 1, 1986
PubMed

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In situ experiments, such as the Doluisio method and Single-Pass Perfusion technique, provide critical insights into drug uptake by simulating in vivo conditions for drug absorption.
The Doluisio method involves perfusing a prepared segment of a rat's small intestine with a solution of radiolabeled drug and a non-absorbable marker. This helps to differentiate between absorbed and non-absorbed drug concentrations. The intestinal segment is connected at both ends using tubing and syringes,...