Determination of aqueous solubility by heating and equilibration: A technical note
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Updated: Mar 6, 2026

Self-Nanoemulsification of Healthy Oils to Enhance the Solubility of Lipophilic Drugs
Published on: July 27, 2022
Thorsteinn Loftsson1, Dagný Hreinsdôttir2
1Faculty of Pharmacy, University of Iceland, Hofsvallagata 53, IS-107, Reykjavik, Iceland. thorstlo@hi.is.
Abstract:
A modified shake-flask solubility method, where the equilibration time was shortened through heating, was used to determine the solubility of 48 different drugs and pharmaceutical excipients in pure water at room temperature. The heating process accelerates dissolution of the solid compound and frequently results in supersaturated solution. Seeding with the solid compound after heating and cooling to room temperature promotes precipitation of the solid compound in its original stable form. This modified shake-flask method generates reliable and reproducible solubility data.
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