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Related Experiment Videos

Presynaptic alpha 2 adrenoreceptor function in dependent rats before and after morphine withdrawal.

H C Moises1, C B Smith, R N Spengler

  • 1Department of Physiology, University of Michigan, Ann Arbor 48109.

NIDA Research Monograph
|January 1, 1986
PubMed
Summary
This summary is machine-generated.

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Morphine dependence alters alpha 2 adrenoreceptor density and function in rat hippocampus. Receptor binding initially decreases then increases post-withdrawal, while presynaptic receptor sensitivity remains impaired, suggesting a role in dependence.

Area of Science:

  • Neuroscience
  • Pharmacology
  • Neurochemistry

Background:

  • Opioid dependence involves complex neuroadaptive changes.
  • Alpha 2 adrenoreceptors play a role in modulating neurotransmitter release.
  • Hippocampal alpha 2 adrenoreceptors are implicated in opioid withdrawal.

Purpose of the Study:

  • To investigate changes in hippocampal alpha 2 adrenoreceptor density and function during morphine dependence and withdrawal.
  • To correlate receptor binding with functional sensitivity in vivo and in vitro.

Main Methods:

  • Quantification of specific [3H]-clonidine binding to hippocampal membranes in morphine-dependent rats and during withdrawal.
  • Assessment of presynaptic alpha 2 adrenoreceptor function using electrically stimulated hippocampal slices and clonidine challenge.

Related Experiment Videos

  • Comparison of receptor density and function at various time points after morphine withdrawal.

    • Main Results:

    • Morphine dependence significantly reduced [3H]-clonidine binding in the hippocampus.
    • Following withdrawal, receptor binding increased, exceeding control levels by 72 hours.
    • Presynaptic alpha 2 adrenoreceptor sensitivity to clonidine was decreased in dependent rats and remained depressed at 72 hours post-withdrawal, despite increased receptor density.

    Conclusions:

    • Changes in hippocampal alpha 2 adrenoreceptor function, particularly presynaptic sensitivity, may be crucial in the development of morphine dependence.
    • Dissociation between receptor density and function suggests complex regulatory mechanisms during opioid withdrawal.