Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Folate and antifolate cell binding and accumulation at physiologic folate concentrations.

B A Kamen1

  • 1Department of Pediatrics, University of Texas Southwestern Medical School, Dallas 75235.

NCI Monographs : a Publication of the National Cancer Institute
|January 1, 1987
PubMed
Summary

Cells utilize a high-affinity folate receptor for nutrient uptake, especially under low folate conditions. This receptor, crucial for cellular folate transport, may serve as a target for new cancer therapies.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

γ-Glutamyl hydrolase modulation and folate influence chemosensitivity of cancer cells to 5-fluorouracil and methotrexate.

British journal of cancer·2013
Same author

Farletuzumab, an anti-folate receptor α antibody, does not block binding of folate or anti-folates to receptor nor does it alter the potency of anti-folates in vitro.

Cancer chemotherapy and pharmacology·2012
Same author

Effects of overexpression of gamma-Glutamyl hydrolase on methotrexate metabolism and resistance.

Cancer research·2001
Same author

High-dose methotrexate is lethal to rats. Why give it to children?

Pediatric hematology and oncology·2000
Same author

Effects of food supplementation and education on folate stores.

Pediatric hematology and oncology·2000
Same author

High-time chemotherapy or high time for low dose.

Journal of clinical oncology : official journal of the American Society of Clinical Oncology·2000

Area of Science:

  • Cell Biology
  • Biochemistry
  • Molecular Medicine

Background:

  • Cellular folate transport traditionally viewed as carrier-mediated or facilitated diffusion.
  • Previous studies used high folic acid concentrations, potentially masking other mechanisms.
  • Folate is essential for cell growth and DNA synthesis.

Purpose of the Study:

  • To investigate folate uptake mechanisms in folate-depleted cells.
  • To identify and characterize the folate receptor involved in cellular uptake.
  • To explore the potential of this receptor as a therapeutic target.

Main Methods:

  • Culturing cells in folate-depleted medium.
  • Measuring folate binding affinities using various folate derivatives and inhibitors.

Related Experiment Videos

  • Utilizing antisera against folate-binding protein (FBP) to block folate cell binding.
  • Assessing the effect of methyltetrahydrofolate incubation on intracellular folate levels and membrane binding.

    • Main Results:

    • Folate-depleted cells exhibited significant high-affinity folate membrane receptor expression.
    • Dissociation constants indicated high affinity for folic acid (0.4 nM) and 5-methyltetrahydrofolate (4 nM).
    • Receptor-mediated uptake was confirmed, with physiological folate concentrations restoring intracellular levels and reducing membrane binding.

    Conclusions:

    • A high-affinity folate receptor mediates cellular folate uptake, functioning at physiological concentrations.
    • This receptor is distinct from simple diffusion or carrier-mediated transport.
    • The folate receptor represents a potential novel membrane target for antifolate and antineoplastic agents.