Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

D-SPIN constructs regulatory network models from scRNA-seq that reveal organizing principles of perturbation response.

Cell·2026

Same author

Clonal amplification of peripheral CD4+ and CD8+ T cells is associated with clinical response to checkpoint blockade in chronic hepatitis B.

ImmunoHorizons·2026

Same author

Single cell RNA-sequencing reveals that testosterone reduces estrogen signaling in the healthy human mammary gland.

Journal of mammary gland biology and neoplasia·2026

Same author

ENS lineage potential is not intrinsically regionalized but is modulated by PTPRZ1 signaling.

bioRxiv : the preprint server for biology·2026

Same author

Stress-relaxing granular bioprinting materials enable complex and uniform organoid self-organization.

Nature materials·2026

Same author

Spatially resolved transcriptome-metabolome integration reveals region-specific glial lipid dysregulation associated with Alzheimer's pathology.

bioRxiv : the preprint server for biology·2026

Same journal

Application of ephrin-B2 loaded glycol chitosan-silk fibroin hydrogel in the treatment of diabetic refractory wounds.

Scientific reports·2026

Same journal

International expert Delphi consensus on thromboprophylaxis in metabolic and bariatric surgery.

Scientific reports·2026

Same journal

Assessing the cross-region knowledge transfer capability of selected deep learning building vectorization methods in the context of available training datasets.

Scientific reports·2026

Same journal

Feasibility and preliminary effects of outdoor versus indoor cognitive-motor therapy in women with Alzheimer's disease: A randomized single-blind pilot study.

Scientific reports·2026

Same journal

Hallmarks of social action in the vocal turn-taking of wild common marmosets (Callithrix jacchus).

Scientific reports·2026

Same journal

Role and mechanism of AOPPs-induced NOX4-mediated ferroptosis in intervertebral disc degeneration.

Scientific reports·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Mar 6, 2026

An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing

An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing

Published on: May 23, 2018

Abseq: Ultrahigh-throughput single cell protein profiling with droplet microfluidic barcoding.

Payam Shahi¹, Samuel C Kim¹, John R Haliburton¹

¹Department of Bioengineering and Therapeutic Sciences and California Institute for Quantitative Biosciences (QB3), University of California, San Francisco, San Francisco, CA 94158, USA.

Scientific Reports

|March 15, 2017

Summary

This summary is machine-generated.

A new method called Abseq uses DNA-tagged antibodies and DNA sequencing to detect and quantify proteins in single cells, overcoming previous limitations in throughput and sensitivity for protein analysis.

More Related Videos

Counting Proteins in Single Cells with Addressable Droplet Microarrays

Counting Proteins in Single Cells with Addressable Droplet Microarrays

Published on: July 6, 2018

Droplet Barcoding-Based Single Cell Transcriptomics of Adult Mammalian Tissues

Droplet Barcoding-Based Single Cell Transcriptomics of Adult Mammalian Tissues

Published on: January 10, 2019

Related Experiment Videos

Last Updated: Mar 6, 2026

An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing

An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing

Published on: May 23, 2018

Counting Proteins in Single Cells with Addressable Droplet Microarrays

Counting Proteins in Single Cells with Addressable Droplet Microarrays

Published on: July 6, 2018

Droplet Barcoding-Based Single Cell Transcriptomics of Adult Mammalian Tissues

Droplet Barcoding-Based Single Cell Transcriptomics of Adult Mammalian Tissues

Published on: January 10, 2019

Area of Science:

Biochemistry
Molecular Biology
Cell Biology

Background:

Proteins are crucial for cellular functions like metabolism and information processing.
Quantifying proteins at the single-cell level is difficult due to limited sample amounts.

Purpose of the Study:

To introduce Abseq, a novel ultrahigh-throughput method for detecting and quantifying proteins in single cells.
To demonstrate the utility of Abseq for characterizing cell surface proteins and distinguishing cell types.

Main Methods:

Abseq utilizes antibodies labeled with DNA sequence tags to detect specific protein epitopes.
Microfluidic barcoding and DNA sequencing are employed for readout of the DNA tags.
This approach allows for amplification of low-abundance tags and quantitative measurements using molecular indices.

Main Results:

Abseq successfully characterized surface proteins on different cell types at the single-cell level.
The method enabled discrimination between cell types based on their unique protein expression profiles.
Demonstrated advantages include amplification of low-abundance signals and high multiplexing capabilities.

Conclusions:

Abseq offers a powerful and scalable solution for single-cell protein analysis.
The method overcomes limitations of existing techniques, enabling deeper insights into cellular heterogeneity.
Abseq has broad applications in cell biology, immunology, and diagnostics.