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Related Concept Videos

Atomic Nuclei: Magnetic Resonance01:05

Atomic Nuclei: Magnetic Resonance

1.3K
The number of nuclear spins aligned in the lower energy state is slightly greater than those in the higher energy state. In the presence of an external magnetic field, as the spins precess at the Larmor frequency, the excess population results in a net magnetization oriented along the z axis. When a pulse or a short burst of radio waves at the Larmor frequency is applied along the x axis, the coupling of frequencies causes resonance and flips the nuclear spins of the excess population from the...
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Atomic Nuclei: Nuclear Relaxation Processes01:23

Atomic Nuclei: Nuclear Relaxation Processes

1.3K
In the absence of an external magnetic field, nuclear spin states are degenerate and randomly oriented. When a magnetic field is applied, the spins begin to precess and orient themselves along (lower energy) or against (higher energy) the direction of the field. At equilibrium, a slight excess population of spins exists in the lower energy state. Because the direction of the magnetic field is fixed as the z-axis,  the precessing magnetic moments are randomly oriented around the z-axis.
1.3K
Two-Dimensional (2D) NMR: Overview01:12

Two-Dimensional (2D) NMR: Overview

1.6K
The 1D NMR spectrum of large and complex molecules like natural products has complicated splitting patterns and overlapping signals, which can be easily interpreted using 2-dimensional (2D) NMR. Unlike 1D NMR, 2D NMR has two frequency axes that provide the coupling information between the nucleus A and nucleus B in a molecule. The process from which 2D spectra are obtained has four steps.
The first step is the preparation period, during which nucleus A is excited with a radiofrequency pulse....
1.6K
Atomic Nuclei: Nuclear Spin State Overview01:03

Atomic Nuclei: Nuclear Spin State Overview

2.1K
NMR-active nuclei have energy levels called 'spin states' that are associated with the orientations of their nuclear magnetic moments. In the absence of a magnetic field, the nuclear magnetic moments are randomly oriented, and the spin states are degenerate. When an external magnetic field is applied, the spin states have only 2 + 1 orientations available to them. A proton with = ½ has two available orientations. Similarly, for a quadrupolar nucleus with a nuclear spin value of one, the...
2.1K
Atomic Nuclei: Nuclear Spin State Population Distribution01:14

Atomic Nuclei: Nuclear Spin State Population Distribution

2.5K
Near absolute zero temperatures, in the presence of a magnetic field, the majority of nuclei prefer the lower energy spin-up state to the higher energy spin-down state. As temperatures increase, the energy from thermal collisions distributes the spins more equally between the two states. The Boltzmann distribution equation gives the ratio of the number of spins predicted in the spin −½ (N−) and spin +½ (N+) states.
2.5K
Atomic Nuclei: Nuclear Magnetic Moment00:59

Atomic Nuclei: Nuclear Magnetic Moment

3.5K
All atomic nuclei are positively charged. When they have a nonzero spin, they behave like rotating charges. As a consequence of their charge and spin, these nuclei generate a magnetic field (B). This, in turn, gives rise to a magnetic moment (μ), which is randomly oriented in the absence of an external magnetic field. When an external magnetic field (B0) is applied, the magnetic moment vectors can align with the field or against it in 2 + 1 orientations. A hydrogen nucleus, which is just a...
3.5K

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Related Experiment Video

Updated: Mar 6, 2026

Atomic Scale Structural Studies of Macromolecular Assemblies by Solid-state Nuclear Magnetic Resonance Spectroscopy
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Atomic Scale Structural Studies of Macromolecular Assemblies by Solid-state Nuclear Magnetic Resonance Spectroscopy

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13C-Decoupled J-Coupling Spectroscopy Using Two-Dimensional Nuclear Magnetic Resonance at Zero-Field.

Tobias F Sjolander1, Michael C D Tayler2,3, Arne Kentner1,4

  • 1Department of Chemistry, University of California at Berkeley , Berkeley, California 94720-3220, United States.

The Journal of Physical Chemistry Letters
|March 15, 2017
PubMed
Summary

This study introduces a novel two-dimensional zero-field NMR method for analyzing proton-proton J-coupling networks. This technique simplifies spectra and enhances analytical capabilities by decoupling carbon-13.

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Area of Science:

  • Nuclear Magnetic Resonance (NMR) Spectroscopy
  • Quantum Spin Dynamics
  • Analytical Chemistry

Background:

  • Zero-field NMR (ZF-NMR) offers unique spectral simplification by eliminating magnetic field-dependent Larmor frequencies.
  • Conventional NMR techniques often face challenges in resolving couplings between chemically equivalent spins.

Purpose of the Study:

  • To develop a two-dimensional method for obtaining 13C-decoupled, 1H-coupled NMR spectra in zero magnetic field.
  • To demonstrate the capability of this method to analyze proton-proton J-coupling networks.
  • To enable the determination of J-coupling constants between chemically equivalent spins.

Main Methods:

  • Utilized coherent spin-decoupling techniques in a zero magnetic field environment.
  • Acquired two-dimensional NMR spectra focusing on proton-proton J-couplings.
  • Applied the method to [1-13C]-propionic acid for spectral analysis.

Main Results:

  • Successfully obtained 13C-decoupled, 1H-coupled zero-field NMR spectra.
  • The resulting spectra are solely determined by the proton-proton J-coupling network.
  • Identified conserved quantum numbers that govern spectral cross-peak appearance.

Conclusions:

  • The developed method simplifies zero-field NMR spectra, enhancing analytical power.
  • This approach allows for direct measurement of J-coupling constants, even for chemically equivalent spins.
  • The findings pave the way for improved structural elucidation using zero-field NMR.