[Inter-and intra-operator variability in the analysis of semen parameters: results from a quality control program]

Area of Science:

• Andrology
• Reproductive Medicine
• Laboratory Quality Management

Background:

• Semen analysis is integral to male infertility diagnostics.
• Ensuring the reliability of andrology laboratory results necessitates robust quality management.
• Variability in semen parameter assessment can impact diagnostic accuracy.

Purpose of the Study:

• To assess intra- and inter-individual variability in semen parameter analysis.
• To evaluate the effectiveness of a quality control program in an andrology laboratory.
• To identify factors influencing variability in semen analysis.

Main Methods:

• Four laboratory participants with varying experience levels analyzed semen samples.
• Sperm motility, concentration, and morphology were assessed.
• Intra-individual variability was determined through replicate sample analysis; inter-individual variability was assessed across participants.

Main Results:

• Inter-participant coefficients of variation were 12.8% (motility), 19.8% (concentration), and 48.9% (morphology).
• Intra-participant coefficients of variation were 6.9% (motility), 12.3% (concentration), and 42.7% (morphology).
• Sperm morphology assessment showed significant influence from participant experience level.

Conclusions:

• Consistent quality control and staff training are essential for reliable semen analysis.
• Regular participation in internal quality control programs enhances laboratory result accuracy.
• Addressing variability, particularly in morphology, is key to improving male infertility investigations.