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Published on: October 14, 2021
MicroRNA expression profiles in human CD3+ T cells following stimulation with anti-human CD3 antibodies
Isabel Garcia Sousa1, Manuela Maragno do Almo1, Kelly Cristina Rodrigues Simi2
1Molecular Pathology Graduation Program, Medicine Faculty, University of Brasilia, Brasilia, Brazil.
Background:
Anti-CD3 therapy can induce immunosuppression by several non mutually exclusive mechanisms that have been proposed to explain the therapeutic effect the administration anti-CD3 mAb, but its immunoregulatory mechanism is still not completely clear. In T cells, microRNAs (miRNAs) regulate several pathways, including those associated with immune tolerance. Here, we report changes in miRNA expression in T cells following treatment with anti-human CD3 antibodies. Peripheral blood mononuclear cells were cultured in the presence of the monoclonal antibody OKT3 or a recombinant fragment of humanized anti-CD3. Following these treatments, the expression profiles of 31 miRNA species were assessed in T cells using TaqMan arrays.
Results:
Eight of the tested miRNAs (miR-155, miR-21, miR-146a, miR-210, miR-17, miR-590-5p, miR-106b and miR-301a) were statistically significantly up- or down-regulated relative to untreated cells.
Conclusions:
Stimulation of T cells with anti-human CD3 antibodies alters miRNA expression patterns, including of miRNA species associated with immune regulatory pathways.
Insights
Anti-CD3 antibody therapy alters microRNA (miRNA) expression in T cells. This finding sheds light on the immunoregulatory mechanisms of anti-CD3 treatment and immune tolerance.
Area of Science:
- Immunology
- Molecular Biology
Background:
- Anti-CD3 antibody therapy is used for immunosuppression, but its precise immunoregulatory mechanisms remain unclear.
- MicroRNAs (miRNAs) are key regulators of T cell pathways involved in immune tolerance.
Purpose of the Study:
- To investigate the impact of anti-human CD3 antibody treatment on miRNA expression profiles in T cells.
Main Methods:
- Peripheral blood mononuclear cells were treated with the anti-CD3 antibody OKT3 or a humanized anti-CD3 fragment.
- TaqMan arrays were used to assess the expression of 31 miRNA species in T cells post-treatment.
Main Results:
- Eight specific miRNAs (miR-155, miR-21, miR-146a, miR-210, miR-17, miR-590-5p, miR-106b, and miR-301a) showed statistically significant differential expression (up- or down-regulation) compared to untreated cells.
Conclusions:
- Anti-human CD3 antibody stimulation significantly alters miRNA expression patterns in T cells.
- These altered miRNA profiles include species known to be involved in immune regulatory pathways.
