Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Prognostic Value of Lung Injury Biomarkers in Patients Hospitalized With COVID-19 Without Respiratory Failure at Admission.

Critical care medicine·2026
Same author

Passive immunotherapy for adults hospitalized with COVID-19: An individual participant data meta-analysis of six randomized controlled trials.

PLoS medicine·2025
Same author

Overcoming myeloid-driven resistance to CAR T therapy by targeting SPP1.

bioRxiv : the preprint server for biology·2025
Same author

Correction: Niche-mediated BMP/SMAD signaling regulates lung alveolar stem cell proliferation and differentiation.

Development (Cambridge, England)·2024
Same author

Clinical Utility of Plasma Microbial Cell-Free DNA Sequencing Among Immunocompromised Patients With Pneumonia.

Open forum infectious diseases·2024
Same author

Author Correction: Cell-type-specific and disease-associated expression quantitative trait loci in the human lung.

Nature genetics·2024

Related Experiment Video

Updated: Mar 6, 2026

Establishing Human Lung Organoids and Proximal Differentiation to Generate Mature Airway Organoids
10:12

Establishing Human Lung Organoids and Proximal Differentiation to Generate Mature Airway Organoids

Published on: March 23, 2022

9.9K

Lung organoids: current uses and future promise.

Christina E Barkauskas1, Mei-I Chung2, Bryan Fioret2

  • 1Department of Medicine, Division of Pulmonary and Critical Medicine, Duke Medicine, Durham, NC 27710, USA christina.barkauskas@duke.edu brigid.hogan@duke.edu.

Development (Cambridge, England)
|March 16, 2017
PubMed
Summary
This summary is machine-generated.

Lung organoids derived from human and mouse cells mimic lung structures. These self-organizing models offer potential for studying lung biology and developing treatments for diseases like cystic fibrosis and asthma.

Keywords:
Lung organoidsLung progenitorsPlasticityStem cells

More Related Videos

Isolation and Enrichment of Human Lung Epithelial Progenitor Cells for Organoid Culture
11:49

Isolation and Enrichment of Human Lung Epithelial Progenitor Cells for Organoid Culture

Published on: July 21, 2020

9.4K
Transplantation of Bioengineered Lung Using Decellularized Mouse Lungs and Primary Human Endothelial Cells
10:13

Transplantation of Bioengineered Lung Using Decellularized Mouse Lungs and Primary Human Endothelial Cells

Published on: March 28, 2025

877

Related Experiment Videos

Last Updated: Mar 6, 2026

Establishing Human Lung Organoids and Proximal Differentiation to Generate Mature Airway Organoids
10:12

Establishing Human Lung Organoids and Proximal Differentiation to Generate Mature Airway Organoids

Published on: March 23, 2022

9.9K
Isolation and Enrichment of Human Lung Epithelial Progenitor Cells for Organoid Culture
11:49

Isolation and Enrichment of Human Lung Epithelial Progenitor Cells for Organoid Culture

Published on: July 21, 2020

9.4K
Transplantation of Bioengineered Lung Using Decellularized Mouse Lungs and Primary Human Endothelial Cells
10:13

Transplantation of Bioengineered Lung Using Decellularized Mouse Lungs and Primary Human Endothelial Cells

Published on: March 28, 2025

877

Area of Science:

  • Pulmonary biology and regenerative medicine.

Background:

  • The lung comprises specialized epithelial cells in conducting airways and alveoli.
  • Certain lung cells, including basal, secretory, and type II cells, can self-organize into three-dimensional organoids in culture.

Purpose of the Study:

  • To review methods for generating lung organoids from human and mouse cells.
  • To compare the structure and cellular makeup of organoids with adult lung tissues.
  • To discuss the utility and limitations of organoids in lung research and drug development.

Main Methods:

  • Isolation of specific lung epithelial cells (basal, secretory, type II).
  • Culturing cells in three-dimensional systems, with or without stromal support.
  • Generation of self-organizing lung organoids.

Main Results:

  • Organoids recapitulate aspects of airway or alveolar structure and cellular composition.
  • Methods for generating lung organoids from human and mouse sources are summarized.
  • Comparison of organoid characteristics to in vivo adult lung tissues is presented.

Conclusions:

  • Lung organoids represent a promising in vitro model system for lung biology research.
  • Organoids hold potential for advancing drug discovery for lung diseases such as cystic fibrosis and asthma.
  • Further research is needed to fully understand and overcome the limitations of current lung organoid models.