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A New Functional Classification of Glucuronoyl Esterases by Peptide Pattern Recognition.

Jane W Agger¹, Peter K Busk¹, Bo Pilgaard¹

¹Center for BioProcess Engineering, Department of Chemical and Biochemical Engineering, Technical University of Denmark Lyngby, Denmark.

Frontiers in Microbiology

|March 16, 2017

Summary

Novel glucuronoyl esterases, crucial for breaking down plant biomass, were identified using bioinformatics. This research expands our understanding of lignocellulose degradation and identifies new enzyme candidates from coprophilic fungi.

Keywords:

CE15 PPR glucuronoxylan glucuronoyl esterase lignin carbohydrate complexes

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Detection of Protease Activity by Fluorescent Peptide Zymography

Detection of Protease Activity by Fluorescent Peptide Zymography

Published on: January 20, 2019

Area of Science:

Biochemistry
Enzymology
Bioinformatics

Background:

Lignocellulosic biomass recalcitrance is partly due to lignin carbohydrate complexes.
Glucuronoyl esterases are enzymes hypothesized to hydrolyze these ester linkages, aiding biomass degradation.
Their precise function and classification within carbohydrate esterases family 15 (CE15) remain uncertain.

Purpose of the Study:

To identify and group novel CE15 proteins with potential glucuronoyl esterase activity using bioinformatic tools.
To analyze the phylogenetic relationships and sequence variations of CE15 proteins.
To explore potential new enzyme functions within the CE15 family and identify promising sources for glucuronoyl esterase discovery.

Main Methods:

Bioinformatic analysis using peptide pattern recognition on 1024 CE15-like sequences from GenBank.
Phylogenetic analysis to group and differentiate CE15 proteins.
Gene annotation in various fungal and bacterial microorganisms.

Main Results:

1024 CE15-like sequences were identified and grouped into 24 distinct clusters.
Phylogenetic analysis revealed putative fungal and bacterial glucuronoyl esterases, along with novel CE15-like sequences.
Coprophilic fungi were identified as particularly rich and diverse sources of CE15 proteins.

Conclusions:

The CE15 family likely encompasses enzymes beyond glucuronoyl esterases, suggesting broader esterase activities.
Coprophilic fungi represent promising candidates for discovering new glucuronoyl esterase genes due to their abundance and diversity of CE15 proteins.
This study provides a foundation for further research into the specific functions and applications of CE15 enzymes in lignocellulose degradation.