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Aptamer-based sandwich-type biosensors.

Ho Bin Seo1, Man Bock Gu1

  • 1Department of Biotechnology, College of Life Sciences and Biotechnology, Korea University, Anam-dong, Seongbuk-Gu, Seoul, 136-713 Republic of Korea.

Journal of Biological Engineering
|March 16, 2017
PubMed
Summary
This summary is machine-generated.

Sandwich-type biosensors offer stable, reproducible, and sensitive detection. This review covers aptamer-aptamer and aptamer-antibody pairs in biosensor designs for various targets.

Keywords:
A pair of aptamerAptamer-antibody complexAptasensorSandwich-type aptasensorSandwich-type biosensors

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Area of Science:

  • Biomedical Engineering
  • Analytical Chemistry
  • Biosensing Technology

Background:

  • Sandwich-type biosensor platforms are recognized for enhanced stability, reproducibility, and sensitivity.
  • These platforms utilize dual receptor pairs that bind to distinct sites on the same target molecule.
  • This approach allows for amplification of the detection signal, improving analytical performance.

Purpose of the Study:

  • To review sandwich-type biosensors employing aptamer-aptamer or aptamer-antibody receptor pairs.
  • To analyze the targets, platforms, schematic designs, and analytical performance of these biosensors.
  • To provide a comprehensive overview of advancements in sandwich-type biosensor technology.

Main Methods:

  • Literature review of scientific publications on sandwich-type biosensors.
  • Categorization of biosensors based on receptor type (aptamer-aptamer, aptamer-antibody).
  • Analysis of reported targets, substrate platforms, assay designs, and performance metrics (e.g., sensitivity, selectivity).

Main Results:

  • Identified diverse applications of sandwich-type biosensors across various targets.
  • Highlighted common schematic designs and innovative platform integrations.
  • Summarized analytical performance data, emphasizing sensitivity enhancements.
  • Aptamer-antibody pairs show broad applicability, while aptamer-aptamer pairs offer specificity.

Conclusions:

  • Sandwich-type biosensors, particularly those using aptamers, represent a powerful tool for sensitive and specific molecular detection.
  • The modular design allows for customization and optimization for a wide range of analytical challenges.
  • Further research can focus on integrating these biosensors into point-of-care devices and complex biological matrices.