Related Experiment Video
Updated: Mar 6, 2026

Studying Protein Import into Chloroplasts Using Protoplasts
Published on: December 10, 2018
Analysis of 1-Aminocyclopropane-1-Carboxylic Acid Uptake Using a Protoplast System
Won-Yong Song1, Sumin Lee2, Moon-Soo Soh3
1Department of Life Science, Pohang University of Science and Technology, Pohang, Republic of Korea.
Abstract:
1-Aminocyclopropane-1-carboxylic acid (ACC) is a biosynthetic precursor of ethylene. The movement of ACC across the plasma membrane (PM) has been implicated in various physiological contexts during environmental adaptation and differentiation in higher plants. A PM-localized transporter in Arabidopsis thaliana, LYSINE HISTIDINE TRANSPORTER1 (LHT1) participates in the uptake of ACC, implicating a class of amino-acid transporters in the transport of ACC. Here, we describe the method for assaying uptake of ACC into the plant cells using an Arabidopsis mesophyll protoplast system.
Related Concept Videos
Protein Transport to the Inner Chloroplast Membrane
Protein Transport to the Outer Chloroplast Membrane
Two models describe the mechanism of precursor recognition and entry across the outer membrane through the TOC complex. Model 1 suggests the newly synthesized precursor binds to the TOC receptor 159 and forms a complex.

