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Identification and characterization of microRNAs in the muscle of Schizothorax prenanti
Runfeng Zhang1, Ruiwen Li2, Yaqiu Lin3
1College of Life Sciences, Hubei Normal University, Huangshi, 435002, China.
Abstract:
MicroRNAs (miRNAs) regulate gene expression by fully or partially binding to complementary sequences and play important roles in skeletal muscle development. However, the roles of miRNAs in the skeletal muscle of Schizothorax prenanti remain unknown. In this study, we analyzed the miRNA profiling in the skeletal muscle of S. prenanti at 30 days post-hatching (dph), 1 year, and 3 years by high-throughput sequencing. Two hundred twenty-nine unique miRNA types aligned to 201 independent pre-miRNA loci according to sequence similarity and 28 novel miRNAs were obtained. The nucleotide bias analysis of identified miRNAs showed that the miRNAs in S. prenanti were highly conserved. Eight identified miRNAs validated using stem-loop qRT-PCR were differentially expressed in the process of skeletal muscle development of S. prenanti. This study provide not only an overall insight into the miRNA landscape in the skeletal muscle of S. prenanti but also a basis for further investigation of miRNA roles in skeletal muscle development of S. prenanti.
Insights
This study reveals microRNAs (miRNAs) involved in skeletal muscle development in Schizothorax prenanti. It identifies conserved and novel miRNAs, providing a foundation for understanding their roles in fish muscle growth.
Area of Science:
- Molecular Biology
- Genomics
- Developmental Biology
Background:
- MicroRNAs (miRNAs) are key regulators of gene expression.
- miRNAs play crucial roles in skeletal muscle development across species.
- The specific roles of miRNAs in Schizothorax prenanti skeletal muscle are currently unknown.
Purpose of the Study:
- To profile microRNAs (miRNAs) in Schizothorax prenanti skeletal muscle at different developmental stages.
- To identify conserved and novel miRNAs involved in skeletal muscle development.
- To provide a basis for future research on miRNA functions in S. prenanti muscle development.
Main Methods:
- High-throughput sequencing was employed to analyze miRNA profiles.
- Skeletal muscle samples were collected from S. prenanti at 30 days post-hatching, 1 year, and 3 years.
- Stem-loop quantitative reverse transcription polymerase chain reaction (qRT-PCR) was used for miRNA validation.
Main Results:
- 229 unique miRNA types and 28 novel miRNAs were identified.
- Nucleotide bias analysis indicated high conservation of miRNAs in S. prenanti.
- Eight validated miRNAs exhibited differential expression during skeletal muscle development.
Conclusions:
- This study presents a comprehensive miRNA landscape for Schizothorax prenanti skeletal muscle.
- Identified miRNAs, including novel ones, are potentially involved in muscle development.
- The findings establish a foundation for elucidating miRNA functions in fish skeletal muscle growth.
