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The Use of a β-lactamase-based Conductimetric Biosensor Assay to Detect Biomolecular Interactions
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Creation of Antigen-Dependent β-Lactamase Fusion Protein Tethered by Circularly Permuted Antibody Variable Domains

Hiroto Iwai1, Miki Kojima-Misaizu1, Jinhua Dong2,3

  • 1Department of Chemistry and Biotechnology, School of Engineering, The University of Tokyo, Bunkyo-ku, Tokyo, 113-8656, Japan.

Methods in Molecular Biology (Clifton, N.J.)
|March 16, 2017
PubMed

Keywords:
Allosteric regulationAntibody variable regionCircular permutationImmunosensorTEM-1 β-lactamase

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