Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Mice lacking two alleles of the schizophrenia risk gene <i>Tcf4</i> and <i>Olig2</i> display deficits in anxiety-related behavior, sensorimotor gating, and cognition.

Frontiers in cellular neuroscience·2026

Same author

Decoding GPCR signaling in living cells to advance early therapeutic discovery for neurological disorders.

Frontiers in molecular neuroscience·2026

Same author

Antagonizing NRG1-ERBB4 signaling pathway with spironolactone for the treatment of schizophrenia: results of a randomized controlled drug repositioning clinical trial.

Communications medicine·2026

Same author

Bridging the Scales via Personalized Cellular Modeling and Deep Phenotyping in Schizophrenia.

JAMA psychiatry·2026

Same author

Protocol for identifying properties of ERBB receptor antagonists using the barcoded ERBBprofiler assay.

STAR protocols·2026

Same author

Personal recovery as outcome measure in mental health research: A meta-analysis on the sensitivity to change properties of the Recovery Assessment Scale.

Journal of affective disorders·2026

Search research articles

Related Experiment Video

Updated: Mar 6, 2026

Split-BioID — Proteomic Analysis of Context-specific Protein Complexes in Their Native Cellular Environment

Split-BioID — Proteomic Analysis of Context-specific Protein Complexes in Their Native Cellular Environment

Published on: April 20, 2018

Characterizing Dynamic Protein-Protein Interactions Using the Genetically Encoded Split Biosensor Assay Technique

Jan P Wintgens¹, Moritz J Rossner¹, Michael C Wehr²

¹Department of Psychiatry, Ludwig Maximilian University of Munich, Nussbaumstr. 7, 80336, Munich, Germany.

Methods in Molecular Biology (Clifton, N.J.)

|March 16, 2017

Summary

Genetically encoded split TEV biosensors monitor dynamic protein-protein interactions (PPIs) in living cells. This robust assay enables studying signaling pathways and drug targets in various cell types for high-throughput screening.

Keywords:

Biosensor Compound profiling Dose-response assay GPCR Phosphorylation-dependent interactions Protein–protein interaction RTK Split TEV Split biosensor assay

More Related Videos

Analyzing Dynamic Protein Complexes Assembled On and Released From Biolayer Interferometry Biosensor Using Mass Spectrometry and Electron Microscopy

Analyzing Dynamic Protein Complexes Assembled On and Released From Biolayer Interferometry Biosensor Using Mass Spectrometry and Electron Microscopy

Published on: August 6, 2018

Use of Recombinant Fusion Proteins in a Fluorescent Protease Assay Platform and Their In-gel Renaturation

Use of Recombinant Fusion Proteins in a Fluorescent Protease Assay Platform and Their In-gel Renaturation

Published on: January 16, 2019

Related Experiment Videos

Last Updated: Mar 6, 2026

Split-BioID — Proteomic Analysis of Context-specific Protein Complexes in Their Native Cellular Environment

Split-BioID — Proteomic Analysis of Context-specific Protein Complexes in Their Native Cellular Environment

Published on: April 20, 2018

Analyzing Dynamic Protein Complexes Assembled On and Released From Biolayer Interferometry Biosensor Using Mass Spectrometry and Electron Microscopy

Analyzing Dynamic Protein Complexes Assembled On and Released From Biolayer Interferometry Biosensor Using Mass Spectrometry and Electron Microscopy

Published on: August 6, 2018

Use of Recombinant Fusion Proteins in a Fluorescent Protease Assay Platform and Their In-gel Renaturation

Use of Recombinant Fusion Proteins in a Fluorescent Protease Assay Platform and Their In-gel Renaturation

Published on: January 16, 2019

Area of Science:

Cellular Biology
Biochemistry
Molecular Biology

Background:

Dynamic protein-protein interactions (PPIs) are crucial for cellular signaling in health and disease.
Understanding PPI regulation is key to deciphering complex biological processes.
Genetically encoded biosensors offer powerful tools for studying PPIs in vivo.

Purpose of the Study:

To present the split TEV method as a versatile tool for monitoring regulated PPIs in living cells.
To highlight the applicability of split TEV for analyzing interactomes and drug targets.
To demonstrate its compatibility with high-throughput screening and various cell types.

Main Methods:

Utilizes the functional complementation of split TEV protease fragments fused to interacting proteins.
Employs genetically encoded readouts, including fluorescence and luminescence reporters.
Integrates with molecular barcode systems for multiplexed, high-throughput assays.

Main Results:

Split TEV provides a robust, sensitive, and flexible readout for PPIs at the membrane and in the cytosol.
The method is effective for analyzing interactomes of receptors, membrane-associated, and cytosolic proteins.
Demonstrates utility in assaying drug targets like receptor tyrosine kinases and G protein-coupled receptors.

Conclusions:

Split TEV is a valuable technique for studying dynamic PPIs and cellular signaling.
Its flexibility and scalability make it suitable for drug discovery and basic research.
Applicable in various cell types, including primary neurons, for broad research use.