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Rapid detection of viral-specific antibodies by enzyme-linked immunosorbent assay (ELISA)
S Winston1, S Fiscus, L Hesterberg
1Allelix Inc., Mississauga, Ontario.
Veterinary Immunology and Immunopathology
|December 1, 1987
Abstract:
The development of three separate rapid ELISAs for detecting antibodies in host serum to three different viruses is described. These include: 1. A direct antigen assay using enzyme labelled anti-canine Ig for detecting antibodies to canine parvovirus, 2. A competitive ELISA using a feline infectious peritonitis virus-specific monoclonal antibody labelled with enzyme, and 3. A competitive ELISA using an equine infectious anemia virus-specific monoclonal antibody and enzyme labelled antigen, p. 26. The utility and benefits of each of the three approaches is emphasized.