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Localization of transfer RNA genes on the physical map of Vibrio eltor phage e4 genome
1Indian Institute of Chemical Biology, Jadavpur, Calcutta.
Abstract:
Transfer RNAs were isolated from uninfected and phage e4-infected Vibrio eltor Mak 757 cells. These tRNAs were then aminoacylated with 3H-labeled amino acids and hybridized to DNA isolated from phage e4. Significant hybridization was observed only with tRNA isolated from phage e4-infected cells. Restriction enzyme digestion of phage e4 DNA followed by Southern blot using [32P]tRNA from infected cells revealed that tRNA genes were contained in a 3.4-kb Kpnl fragment. The tRNA genes were located on the physical map of the phage genome 19 kb from one of the termini.
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