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Lactococcus lactis KR-050L inhibit IL-6/STAT3 activation.

J T Hwang1,2, H-J Jang1, J H Kim1

  • 1Natural Product Research Center, Korea Research Institute of Bioscience and Biotechnology, 181 Ipsin-gil, Jeongeup-si, Jeonbuk, 56212, Korea.

Journal of Applied Microbiology
|March 16, 2017
PubMed
Summary
This summary is machine-generated.

Researchers isolated lactic acid bacteria (LABs) from kimchi, identifying Lactococcus lactis KR-050L as a source of compounds that inhibit Interleukin-6/Signal Transducer and Activator of Transcription 3 (IL-6/STAT3) signaling.

Keywords:
2,5-diketopipperazineGajuknamu kimchiIL-6Lactococcus lactis KR-050LSTAT3

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Area of Science:

  • Microbiology
  • Biochemistry
  • Pharmacology

Background:

  • Interleukin-6 (IL-6) and Signal Transducer and Activator of Transcription 3 (STAT3) signaling pathways are implicated in various diseases.
  • Lactic acid bacteria (LABs) are known for their diverse biological activities and potential health benefits.
  • Kimchi, a fermented food, is a rich source of various microorganisms, including LABs.

Purpose of the Study:

  • To isolate and identify LABs from Gajuknamu kimchi.
  • To investigate the IL-6/STAT3 inhibitory activity of these LABs and their derived compounds.
  • To explore the potential of these LABs and compounds as functional food ingredients for disease treatment.

Main Methods:

  • Isolation and 16S rRNA sequencing of LABs from Gajuknamu kimchi.
  • Assay of IL-6-induced STAT3 luciferase activity using LAB culture broths.
  • Isolation and structural elucidation of compounds from Lactococcus lactis KR-050L using chromatography and spectroscopic analyses (MS, 1H- and 13C-NMR).

Main Results:

  • Six LABs were isolated, with Lactococcus lactis KR-050L showing significant inhibition of IL-6-induced STAT3 activity.
  • Fifteen 2,5-diketopiperazine (2,5-DKP) compounds were isolated from Lc. lactis KR-050L.
  • The isolated 2,5-DKPs demonstrated inhibitory activity against IL-6/STAT3 activation, with activity influenced by structural features like phenylalanine residues and hydroxyl groups.

Conclusions:

  • Lactococcus lactis KR-050L was identified as a promising source of IL-6/STAT3 inhibitors.
  • The study provides the first report of isolating LABs from Gajuknamu kimchi and discovering IL-6/STAT3 inhibitors from Lc. lactis KR-050L.
  • The findings support the potential commercialization of Lc. lactis from Gajuknamu kimchi as a functional food for managing diseases associated with IL-6/STAT3 activation.