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Polyoma integrates readily in mouse cellular DNA.

G Roy1, P Chartrand

  • 1Département de Microbiologie, Faculté de Médecine, Université de Sherbrooke, Québec, Canada.

Virus Research
|January 1, 1988
PubMed
Summary
This summary is machine-generated.

Polyoma virus readily integrates into mouse cells, unlike previous studies in rat cells. The large T protein is key to this integration, even without viral replication.

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Area of Science:

  • Virology
  • Molecular Biology
  • Genetics

Background:

  • Mouse polyomavirus naturally infects mice.
  • Previous studies on polyomavirus integration focused on rat cells, not its natural host.

Purpose of the Study:

  • To investigate polyomavirus integration in mouse cells.
  • To determine the role of the large T protein in polyomavirus integration.

Main Methods:

  • Cotransfection of mouse cell lines with the polyomavirus genome and selectable markers (tk or neo genes).
  • Analysis of TK+ or G418R clones to assess integration efficiency.
  • Investigating the necessity and sufficiency of the large T protein for integration.

Main Results:

  • Polyomavirus presence reduced the number of selectable clones by up to 50-fold.
  • The large T protein was necessary and sufficient for this reduction.
  • All analyzed clones (33/33) surviving cotransfection contained integrated polyomavirus DNA.
  • Over 50% of these clones showed integration of the entire polyomavirus genome.
  • Integration occurred independently of polyomavirus replication.

Conclusions:

  • Polyomavirus DNA integrates readily into mouse cellular DNA.
  • The large T protein plays a crucial role in facilitating polyomavirus integration in its natural host.