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Related Experiment Video

Updated: Mar 6, 2026

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Quantification of piritramide in human colostrum.

A Van Eeckhaut1, P Vanfleteren2, J Van Schoors1

  • 1Department of Pharmaceutical Chemistry and Drug Analysis, Center for Pharmaceutical Research (CePhar), Vrije Universiteit Brussel, Brussels, Belgium.

Journal of Clinical Pharmacy and Therapeutics
|March 16, 2017
PubMed
Summary
This summary is machine-generated.

This study developed a method to detect piritramide in breast milk after Cesarean sections. Piritramide levels in colostrum were found to be low, mostly below the quantification limit.

Keywords:
alkaline pHcolostrumliquid chromatographymixed-mode cation exchangepiritramidesolid-phase extraction

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Area of Science:

  • Pharmacology
  • Analytical Chemistry
  • Obstetrics

Background:

  • Post-operative pain management after Cesarean sections often involves piritramide via patient-controlled intravenous analgesia (PCIA).
  • Limited data exists on piritramide transfer into breast milk, leading to breastfeeding restrictions for mothers receiving this analgesic.

Purpose of the Study:

  • To develop and validate a sensitive liquid chromatography (LC) method for quantifying piritramide in colostrum.
  • To assess the presence of piritramide in the first milk of mothers who received PCIA for post-Cesarean pain control.

Main Methods:

  • Piritramide was isolated and concentrated from colostrum using protein precipitation and solid-phase extraction (SPE) with a mixed-mode cation exchange sorbent.
  • Analysis was performed using reversed-phase LC with UV detection on a microbore C18 column and an alkaline mobile phase.

Main Results:

  • The developed LC-UV method demonstrated good validation characteristics including linearity, accuracy, precision, and stability.
  • Analysis of colostrum samples from ten patients revealed low concentrations of piritramide, predominantly below the limit of quantification (30 ng/mL).

Conclusions:

  • This is the first reported method for quantifying piritramide in colostrum.
  • The findings suggest that piritramide administered via PCIA results in minimal transfer to the first breast milk, potentially allowing breastfeeding.