Endometrial biopsy in Bos indicus beef heifers
O Ramirez-Garzon1, N Satake1, R E Lyons1
1School of Veterinary Science, University of Queensland, Gatton, Australia.
Reproduction in Domestic Animals = Zuchthygiene
|March 16, 2017
Summary
A novel, smaller human bronchoscopy biopsy device successfully collected endometrial biopsies in 86% of beef heifers, enabling uterine environment assessment for reproductive research.
Area of Science:
- Veterinary Science
- Reproductive Biology
- Animal Science
Background:
- Endometrial biopsies are crucial for assessing the uterine environment in breeding females.
- Existing biopsy devices are unsuitable for heifers due to cervical anatomy.
Purpose of the Study:
- To evaluate a human bronchoscopy biopsy device for endometrial sampling in Bos indicus beef heifers.
- To assess the feasibility and quality of endometrial biopsies obtained using this device.
Main Methods:
- A Karl Storz® 10366L human bronchoscopy biopsy device was utilized.
- Endometrial biopsies were attempted in 51 Bos indicus beef heifers.
- Tissue quality, RNA yield, and RNA integrity number (RIN) were assessed.
Main Results:
- The device enabled endometrial biopsy collection in 86% (44/51) of heifers.
- Biopsied tissue was of good quality, suitable for transcriptomic analysis.
- Average total RNA yield was 1.3 μg (range 0.4-5.3 μg) with an average RIN of 7.4 (range 5.7-8.4).
Conclusions:
- The human bronchoscopy biopsy device is a practical alternative for obtaining endometrial biopsies in heifers.
- This method facilitates transcriptomic assessment of the bovine endometrium, advancing reproductive research.
